CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008666
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 PAB1-binding protein 1 
Protein Synonyms/Alias
  
Gene Name
 PBP1 
Gene Synonyms/Alias
 MRS16; YGR178C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
170GESNEVTKFRTDVDIacetylation[1]
191IKERKLEKWTPEEGAacetylation[1]
260QEAERIAKEIESQGTacetylation[1]
344ISSSNSNKNENAVSTubiquitination[2]
433ISKTPSAKTVSPTTQacetylation[3]
433ISKTPSAKTVSPTTQubiquitination[4]
445TTQISAGKSESRRSGubiquitination[4]
484HGSFFGAKNPHTNDAacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] mChIP-KAT-MS, a method to map protein interactions and acetylation sites for lysine acetyltransferases.
 Mitchell L, Huard S, Cotrut M, Pourhanifeh-Lemeri R, Steunou AL, Hamza A, Lambert JP, Zhou H, Ning Z, Basu A, Côté J, Figeys DA, Baetz K.
 Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1641-50. [PMID: 23572591]
 [4] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Appears to promote proper polyadenylation. In the absence of PBP1, the 3'termini of pre-mRNAs are properly cleaved but lack full-length poly(A) tails. May act to repress the ability of PAB1 to negatively regulate polyadenylation. Negative regulator of poly(A) nuclease (PAN) activity. 
Sequence Annotation
 MOD_RES 106 106 Phosphoserine.
 MOD_RES 193 193 Phosphothreonine.
 MOD_RES 215 215 Phosphoserine.
 MOD_RES 436 436 Phosphoserine.  
Keyword
 Complete proteome; Cytoplasm; Mitochondrion; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 722 AA 
Protein Sequence
MKGNFRKRDS STNSRKGGNS DSNYTNGGVP NQNNSSMFYE NPEITRNFDD RQDYLLANSI 60
GSDVTVTVTS GVKYTGLLVS CNLESTNGID VVLRFPRVAD SGVSDSVDDL AKTLGETLLI 120
HGEDVAELEL KNIDLSLDEK WENSKAQETT PARTNIEKER VNGESNEVTK FRTDVDISGS 180
GREIKERKLE KWTPEEGAEH FDINKGKALE DDSASWDQFA VNEKKFGVKS TFDEHLYTTK 240
INKDDPNYSK RLQEAERIAK EIESQGTSGN IHIAEDRGII IDDSGLDEED LYSGVDRRGD 300
ELLAALKSNS KPNSNKGNRY VPPTLRQQPH HMDPAIISSS NSNKNENAVS TDTSTPAAAG 360
APEGKPPQKT SKNKKSLSSK EAQIEELKKF SEKFKVPYDI PKDMLEVLKR SSSTLKSNSS 420
LPPKPISKTP SAKTVSPTTQ ISAGKSESRR SGSNISQGQS STGHTTRSST SLRRRNHGSF 480
FGAKNPHTND AKRVLFGKSF NMFIKSKEAH DEKKKGDDAS ENMEPFFIEK PYFTAPTWLN 540
TIEESYKTFF PDEDTAIQEA QTRFQQRQLN SMGNAVPGMN PAMGMNMGGM MGFPMGGPSA 600
SPNPMMNGFA AGSMGMYMPF QPQPMFYHPS MPQMMPVMGS NGAEEGGGNI SPHVPAGFMA 660
AGPGAPMGAF GYPGGIPFQG MMGSGPSGMP ANGSAMHSHG HSRNYHQTSH HGHHNSSTSG 720
HK 722 
Gene Ontology
 GO:0010494; C:cytoplasmic stress granule; IDA:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0005844; C:polysome; IDA:SGD.
 GO:0006378; P:mRNA polyadenylation; IPI:SGD.
 GO:0045727; P:positive regulation of translation; IPI:SGD.
 GO:0034063; P:stress granule assembly; IMP:SGD. 
Interpro
 IPR009604; LsmAD_domain.
 IPR025852; SM_dom_ATX. 
Pfam
 PF06741; LsmAD
 PF14438; SM-ATX 
SMART
  
PROSITE
  
PRINTS