UniProt Accession

 ADAM9_HUMAN; Q13443; B7ZLN7; Q10718; Q8NFM6 

Genbank Protein ID

 U41766; AF495383; D14665; CH471080; BC143923 

Genbank Nucleotide ID

 AAC50403.1; AAM49575.1; BAA03499.2; EAW63284.1; AAI43924.1 

Protein Name

 Disintegrin and metalloproteinase domain-containing protein 9 

Protein Synonyms/Alias

 ADAM 9; Cellular disintegrin-related protein; Meltrin-gamma; Metalloprotease/disintegrin/cysteine-rich protein 9; Myeloma cell metalloproteinase 

Gene Name

 ADAM9 

Gene Synonyms/Alias

 KIAA0021; MCMP; MDC9; MLTNG 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
741	QTYESDGKNQANPSR	ubiquitination	[1, 2, 3, 4]
781	AVPTYAAKQPQQFPS	ubiquitination	[1, 4, 5]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 

Functional Description

 Probable zinc protease. May mediate cell-cell or cell- matrix interactions. Isoform 2 displays alpha-secretase activity for APP. 

Sequence Annotation

 DOMAIN 212 406 Peptidase M12B.
 DOMAIN 414 501 Disintegrin.
 DOMAIN 644 698 EGF-like.
 ACT_SITE 348 348 By similarity.
 METAL 347 347 Zinc; catalytic (By similarity).
 METAL 351 351 Zinc; catalytic (By similarity).
 METAL 357 357 Zinc; catalytic (By similarity).
 CARBOHYD 125 125 N-linked (GlcNAc...) (Potential).
 CARBOHYD 144 144 N-linked (GlcNAc...) (Potential).
 CARBOHYD 154 154 N-linked (GlcNAc...) (Potential).
 CARBOHYD 231 231 N-linked (GlcNAc...) (Potential).
 CARBOHYD 381 381 N-linked (GlcNAc...) (Potential).
 CARBOHYD 487 487 N-linked (GlcNAc...) (Potential).
 DISULFID 322 401 By similarity.
 DISULFID 363 385 By similarity.
 DISULFID 365 370 By similarity.
 DISULFID 473 493 By similarity.
 DISULFID 644 656 By similarity.
 DISULFID 650 662 By similarity.
 DISULFID 664 673 By similarity.  

Keyword

 3D-structure; Alternative splicing; Cell membrane; Complete proteome; Cone-rod dystrophy; Disulfide bond; Glycoprotein; Hydrolase; Membrane; Metal-binding; Metalloprotease; Protease; Reference proteome; Secreted; Signal; Transmembrane; Transmembrane helix; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 819 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0005615; C:extracellular space; IDA:BHF-UCL.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0031233; C:intrinsic to external side of plasma membrane; ISS:BHF-UCL.
 GO:0005518; F:collagen binding; IMP:BHF-UCL.
 GO:0005178; F:integrin binding; IDA:BHF-UCL.
 GO:0043236; F:laminin binding; IMP:BHF-UCL.
 GO:0004222; F:metalloendopeptidase activity; IDA:BHF-UCL.
 GO:0005080; F:protein kinase C binding; ISS:BHF-UCL.
 GO:0017124; F:SH3 domain binding; ISS:BHF-UCL.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0000186; P:activation of MAPKK activity; IDA:BHF-UCL.
 GO:0033631; P:cell-cell adhesion mediated by integrin; IEP:BHF-UCL.
 GO:0007160; P:cell-matrix adhesion; IMP:BHF-UCL.
 GO:0071222; P:cellular response to lipopolysaccharide; IMP:BHF-UCL.
 GO:0030574; P:collagen catabolic process; TAS:Reactome.
 GO:0022617; P:extracellular matrix disassembly; TAS:Reactome.
 GO:0007229; P:integrin-mediated signaling pathway; IC:BHF-UCL.
 GO:0030216; P:keratinocyte differentiation; IEP:BHF-UCL.
 GO:0042117; P:monocyte activation; IMP:BHF-UCL.
 GO:0051088; P:PMA-inducible membrane protein ectodomain proteolysis; IDA:BHF-UCL.
 GO:0033630; P:positive regulation of cell adhesion mediated by integrin; IMP:BHF-UCL.
 GO:0051549; P:positive regulation of keratinocyte migration; IMP:BHF-UCL.
 GO:0034241; P:positive regulation of macrophage fusion; IMP:BHF-UCL.
 GO:0051044; P:positive regulation of membrane protein ectodomain proteolysis; ISS:BHF-UCL.
 GO:0050714; P:positive regulation of protein secretion; IDA:BHF-UCL.
 GO:0051592; P:response to calcium ion; IMP:BHF-UCL.
 GO:0051384; P:response to glucocorticoid stimulus; ISS:BHF-UCL.
 GO:0042542; P:response to hydrogen peroxide; IMP:BHF-UCL.
 GO:0010042; P:response to manganese ion; IMP:BHF-UCL.
 GO:0034612; P:response to tumor necrosis factor; IDA:BHF-UCL.
 GO:0007179; P:transforming growth factor beta receptor signaling pathway; IMP:BHF-UCL. 

Interpro

 IPR006586; ADAM_Cys-rich.
 IPR001762; Blood-coag_inhib_Disintegrin.
 IPR018358; Disintegrin_CS.
 IPR000742; EG-like_dom.
 IPR013032; EGF-like_CS.
 IPR024079; MetalloPept_cat_dom.
 IPR001590; Peptidase_M12B.
 IPR002870; Peptidase_M12B_N. 

Pfam

 PF08516; ADAM_CR
 PF00200; Disintegrin
 PF01562; Pep_M12B_propep
 PF01421; Reprolysin 

SMART

 SM00608; ACR
 SM00050; DISIN
 SM00181; EGF 

PROSITE

 PS50215; ADAM_MEPRO
 PS00427; DISINTEGRIN_1
 PS50214; DISINTEGRIN_2
 PS00022; EGF_1
 PS01186; EGF_2
 PS50026; EGF_3
 PS00142; ZINC_PROTEASE 

PRINTS