CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017472
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ADP-ribosylation factor GTPase-activating protein 2 
Protein Synonyms/Alias
 ARF GAP 2; GTPase-activating protein ZNF289; Zinc finger protein 289 
Gene Name
 ARFGAP2 
Gene Synonyms/Alias
 ZNF289; Nbla10535 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
7*MAAEPNKTEIQTLFubiquitination[1]
15TEIQTLFKRLRAVPTubiquitination[1]
24LRAVPTNKACFDCGAubiquitination[1]
209PKASLELKSSIIGKKubiquitination[1]
336QETPVSAKSSRSQLDubiquitination[1]
357TFASGPPKYKDNPFSubiquitination[1]
359ASGPPKYKDNPFSLGubiquitination[1]
429RQKFAGAKAISSDMFubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Implicated in coatomer-mediated protein transport between the Golgi complex and the endoplasmic reticulum. Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes. 
Sequence Annotation
 DOMAIN 11 127 Arf-GAP.
 ZN_FING 26 49 C4-type.
 REGION 97 521 Required for interaction with coatomer.
 MOD_RES 146 146 Phosphoserine.
 MOD_RES 337 337 Phosphoserine (By similarity).
 MOD_RES 368 368 Phosphoserine.
 MOD_RES 432 432 Phosphoserine.
 MOD_RES 433 433 Phosphoserine.  
Keyword
 3D-structure; Coiled coil; Complete proteome; Cytoplasm; ER-Golgi transport; Golgi apparatus; GTPase activation; Membrane; Metal-binding; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Transport; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 521 AA 
Protein Sequence
MAAEPNKTEI QTLFKRLRAV PTNKACFDCG AKNPSWASIT YGVFLCIDCS GVHRSLGVHL 60
SFIRSTELDS NWNWFQLRCM QVGGNANATA FFRQHGCTAN DANTKYNSRA AQMYREKIRQ 120
LGSAALARHG TDLWIDNMSS AVPNHSPEKK DSDFFTEHTQ PPAWDAPATE PSGTQQPAPS 180
TESSGLAQPE HGPNTDLLGT SPKASLELKS SIIGKKKPAA AKKGLGAKKG LGAQKVSSQS 240
FSEIERQAQV AEKLREQQAA DAKKQAEESM VASMRLAYQE LQIDRKKEEK KLQNLEGKKR 300
EQAERLGMGL VSRSSVSHSV LSEMQVIEQE TPVSAKSSRS QLDLFDDVGT FASGPPKYKD 360
NPFSLGESFG SRWDTDAAWG MDRVEEKEPE VTISSIRPIS ERATNRREVE SRSSGLESSE 420
ARQKFAGAKA ISSDMFFGRE VDAEYEARSR LQQLSGSSAI SSSDLFGDMD GAHGAGSVSL 480
GNVLPTADIA QFKQGVKSVA GKMAVLANGV MNSLQDRYGS Y 521 
Gene Ontology
 GO:0005794; C:Golgi apparatus; IDA:HPA.
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0008060; F:ARF GTPase activator activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0043547; P:positive regulation of GTPase activity; IEA:GOC.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0032312; P:regulation of ARF GTPase activity; IEA:InterPro.
 GO:0016192; P:vesicle-mediated transport; IEA:UniProtKB-KW. 
Interpro
 IPR001164; ArfGAP. 
Pfam
 PF01412; ArfGap 
SMART
 SM00105; ArfGap 
PROSITE
 PS50115; ARFGAP 
PRINTS
 PR00405; REVINTRACTNG.