CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024223
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA gyrase subunit B-like protein MSMEG_0457/MSMEI_0444 
Protein Synonyms/Alias
  
Gene Name
 MSMEG_0457, MSMEI_0444 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 
NCBI Taxa ID
 246196 
Lysine Modification
Position
Peptide
Type
References
408DTVTLWSKLALESARpupylation[1]
Reference
 [1] Expansion of the mycobacterial "PUPylome".
 Watrous J, Burns K, Liu WT, Patel A, Hook V, Bafna V, Barry CE 3rd, Bark S, Dorrestein PC.
 Mol Biosyst. 2010 Feb;6(2):376-85. [PMID: 20094657] 
Functional Description
  
Sequence Annotation
 DOMAIN 458 572 Toprim.
 CROSSLNK 408 408 Isoglutamyl lysine isopeptide (Lys-Gln)  
Keyword
 ATP-binding; Complete proteome; Isomerase; Isopeptide bond; Nucleotide-binding; Reference proteome; Topoisomerase; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 677 AA 
Protein Sequence
MSYTAADITE LDDVQHTRLR PAVNLGLDVL NTALREIVDN AIEEVADPGH GGSTVTITLH 60
ADGSVSVADD GRGLPVDTDP TTGKNGIVKT LGTARAGGKF SAHKDATSTG AGLNGIGAAA 120
AVFISARTDV TVRRDGKTFL QSFGRGYPGV FEGKEFDPEA PFTRNDTQKL RGVSNRKPDL 180
HGTEVRILFD PAIAPDSTLD IGEVLLRAHA AARMSPGVHL VVVDEGWPGE EVPPAVLEPF 240
SGPWGTDTLL DLMCTAAGTP LPEVRAVVEG RGEYTTGRGP TPFRWSLTAG PAEPATVAAF 300
CNTVRTPGGG SHLTAAIKGL SEALAERASR MRDLGLAKNE EGPEPQDFAA VTALAVDTRA 360
PDVAWDSQAK TAVSSRSLNL AMAPDVARSV TIWAANPANA DTVTLWSKLA LESARARRSA 420
EGAKARARAA SKAKGLGTNL SLPPKLLPSR ESGRGSGAEL FLCEGDSALG TIKAARDATF 480
QAAFPLKGKP PNVYGFPLNK ARAKDEFDAI ERILGCGVRD HCDPELCRYD RILFASDADP 540
DGGNINSSLI SMFLDFYRPL VEAGMVYVTM PPLFVVKAGD ERIYCQDESE RDAAVAQLKA 600
SSNRRVEVQR NKGLGEMDAD DFWNTVLDPQ RRTVIRVRPD ESEKKLHHTL FGGPPEGRRT 660
WMADVAARVD TSALDLT 677 
Gene Ontology
 GO:0005694; C:chromosome; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003918; F:DNA topoisomerase type II (ATP-hydrolyzing) activity; IEA:InterPro.
 GO:0006265; P:DNA topological change; IEA:InterPro. 
Interpro
 IPR002288; DNA_gyrase_B_C.
 IPR003594; HATPase_ATP-bd.
 IPR020568; Ribosomal_S5_D2-typ_fold.
 IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
 IPR001241; Topo_IIA.
 IPR013506; Topo_IIA_bsu_dom2.
 IPR013759; Topo_IIA_cen_dom.
 IPR013760; Topo_IIA_like_dom.
 IPR018522; TopoIIA_CS.
 IPR006171; Toprim_domain. 
Pfam
 PF00204; DNA_gyraseB
 PF00986; DNA_gyraseB_C
 PF02518; HATPase_c
 PF01751; Toprim 
SMART
 SM00387; HATPase_c
 SM00433; TOP2c 
PROSITE
 PS00177; TOPOISOMERASE_II
 PS50880; TOPRIM 
PRINTS
 PR00418; TPI2FAMILY.