UniProt Accession

 SPS1_HUMAN; P49903; B4DWK0; D3DRS9; D6PSQ9; Q5T5U8; Q5T5U9; Q9BVT4 

Genbank Protein ID

 U34044; GU954545; GU954546; GU954547; GU954548; GU954549; AK301568; AL138764; AL355870; AL355870; AL138764; CH471072; CH471072; CH471072; CH471072; CH471072; BC000941; BC063816 

Genbank Nucleotide ID

 AAA87567.1; ADF78120.1; ADF78121.1; ADF78122.1; ADF78123.1; ADF78124.1; BAG63062.1; CAI12907.1; CAI12907.1; CAI14198.1; CAI14198.1; EAW86289.1; EAW86290.1; EAW86291.1; EAW86292.1; EAW86293.1; AAH00941.1; AAH63816.1 

Protein Name

 Selenide, water dikinase 1 

Protein Synonyms/Alias

 Selenium donor protein 1; Selenophosphate synthase 1 

Gene Name

 SEPHS1 

Gene Synonyms/Alias

 SELD; SPS; SPS1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
16	PESYELDKSFRLTRF	ubiquitination	[1, 2, 3]
27	LTRFTELKGTGCKVP	acetylation	[3]
27	LTRFTELKGTGCKVP	ubiquitination	[2]
139	MTDRERDKVMPLIIQ	ubiquitination	[1, 2, 3, 4, 5]
341	ARFCAEIKSPKYGEG	acetylation	[6]
341	ARFCAEIKSPKYGEG	ubiquitination	[2, 3]

Reference

 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 

Functional Description

 Synthesizes selenophosphate from selenide and ATP. 

Sequence Annotation

 NP_BIND 67 69 ATP.
 NP_BIND 162 164 ATP; shared with dimeric partner.
 NP_BIND 267 273 ATP.
 ACT_SITE 31 31 Potential.
 BINDING 32 32 ATP.
 BINDING 87 87 ATP.
 BINDING 110 110 ATP.
 MOD_RES 2 2 N-acetylserine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; ATP-binding; Cell membrane; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Membrane; Nucleotide-binding; Nucleus; Reference proteome; Selenium; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 392 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0031965; C:nuclear membrane; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; IDA:UniProtKB.
 GO:0005524; F:ATP binding; TAS:ProtInc.
 GO:0005525; F:GTP binding; TAS:ProtInc.
 GO:0046982; F:protein heterodimerization activity; IDA:UniProtKB.
 GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
 GO:0004756; F:selenide, water dikinase activity; IEA:EC.
 GO:0006464; P:cellular protein modification process; TAS:ProtInc. 

Interpro

 IPR010918; AIR_synth_C_dom.
 IPR000728; AIR_synth_N_dom.
 IPR016188; PurM_N-like.
 IPR004536; SelD. 

Pfam

 PF00586; AIRS
 PF02769; AIRS_C 

SMART

  

PROSITE

  

PRINTS