CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011611
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcription factor GAGA 
Protein Synonyms/Alias
 Adh transcription factor 2; GAGA factor; GAF; Neural conserved at 70F; Trithorax-like protein 
Gene Name
 Trl 
Gene Synonyms/Alias
 Adf-2; GAGA; Nc70F; TFGAGA; CG33261 
Created Date
 July 27, 2013 
Organism
 Drosophila melanogaster (Fruit fly) 
NCBI Taxa ID
 7227 
Lysine Modification
Position
Peptide
Type
References
325TPKAKRAKHPPGTEKacetylation[1]
373HFAKPGVKKEKKTTSacetylation[1]
Reference
 [1] Acetylation of GAGA factor modulates its interaction with DNA.
 Aran-Guiu X, Ortiz-Lombardía M, Oliveira E, Bonet Costa C, Odena MA, Bellido D, Bernués J.
 Biochemistry. 2010 Nov 2;49(43):9140-51. [PMID: 20849112] 
Functional Description
 Transcriptional activator that functions by regulating chromatin structure. Overcomes the repressive effects of chromatin by promoting the open chromatin conformation in promoter gene regions, thereby allowing access to other transcription factors. Binds to DNA Polycomb response elements (PREs) at the bithorax complex and to the proximal region of the engrailed promoter, and positively regulates transcription of many genes including homeotic ones. Binds to the DNA sequence (GA)n, with optimal binding to the pentamer 5'-GAGAG-3'. Binds DNA as an oligomer. May also act as a transcriptional repressor, maintaining the repressed state of genes including lolal, and down-regulating its own transcription. Required for dosage compensation in males and may be involved in oogenesis. Also has a role in nuclear division. 
Sequence Annotation
 DOMAIN 34 99 BTB.
 ZN_FING 343 366 C2H2-type; degenerate.
 REGION 201 397 Interaction with E(bx).
 MOD_RES 237 237 Phosphothreonine.  
Keyword
 3D-structure; Activator; Alternative splicing; Cell cycle; Cell division; Chromatin regulator; Complete proteome; Developmental protein; Differentiation; Direct protein sequencing; DNA-binding; Metal-binding; Mitosis; Nucleus; Oogenesis; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 581 AA 
Protein Sequence
MSLPMNSLYS LTWGDYGTSL VSAIQLLRCH GDLVDCTLAA GGRSFPAHKI VLCAASPFLL 60
DLLKNTPCKH PVVMLAGVNA NDLEALLEFV YRGEVSVDHA QLPSLLQAAQ CLNIQGLAPQ 120
TVTKDDYTTH SIQLQHMIPQ HHDQDQLIAT IATAPQQTVH AQVVEDIHHQ GQILQATTQT 180
NAAGQQQTIV TTDAAKHDQA VIQAFLPARK RKPRVKKMSP TAPKISKVEG MDTIMGTPTS 240
SHGSGSVQQV LGENGAEGQL LSSTPIIKSE GQKVETIVTM DPNNMIPVTS ANAATGEITP 300
AQGATGSSGG NTSGVLSTPK AKRAKHPPGT EKPRSRSQSE QPATCPICYA VIRQSRNLRR 360
HLELRHFAKP GVKKEKKTTS GKKSSSGSSG SGSGALSSSG SVPQVQTVQS LHTLQGVQVK 420
KDPDAQQQQQ QQQQQQQQQQ QAMTVSGATG GQVQQQVQQV QQQVQQQQQQ QQQQQQQLQH 480
HQIIDSSGNI TTATTSAQAA AAAQQQAAGQ QQQLVAQSDG SESGAPLSIA QVQTLQGHQI 540
IGNLNQVNMT DFQQQQPQQQ QQQQQQQQQQ QQQQQQTQQT L 581 
Gene Ontology
 GO:0005721; C:centromeric heterochromatin; IDA:FlyBase.
 GO:0000791; C:euchromatin; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005700; C:polytene chromosome; IDA:UniProtKB.
 GO:0003682; F:chromatin binding; IDA:FlyBase.
 GO:0043565; F:sequence-specific DNA binding; IDA:FlyBase.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IMP:FlyBase.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0031497; P:chromatin assembly; IDA:UniProtKB.
 GO:0016568; P:chromatin modification; IEA:UniProtKB-KW.
 GO:0007549; P:dosage compensation; IMP:FlyBase.
 GO:0007476; P:imaginal disc-derived wing morphogenesis; IMP:FlyBase.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IGI:FlyBase.
 GO:0000280; P:nuclear division; IMP:FlyBase.
 GO:0048477; P:oogenesis; IEA:UniProtKB-KW.
 GO:0031937; P:positive regulation of chromatin silencing; IDA:FlyBase.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IMP:FlyBase.
 GO:0051259; P:protein oligomerization; IDA:UniProtKB.
 GO:0035186; P:syncytial blastoderm mitotic cell cycle; IMP:FlyBase.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR013069; BTB_POZ.
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd.
 IPR015318; Znf_GAGA-bd_fac. 
Pfam
 PF00651; BTB
 PF09237; GAGA 
SMART
 SM00225; BTB
 SM00355; ZnF_C2H2 
PROSITE
 PS50097; BTB
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS