CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024334
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Activating molecule in BECN1-regulated autophagy protein 1 
Protein Synonyms/Alias
  
Gene Name
 Ambra1 
Gene Synonyms/Alias
 Kiaa1736 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
175REPFAVVKTASEMERubiquitination[1, 2]
Reference
 [1] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation. 
Sequence Annotation
 REPEAT 51 90 WD 1.
 REPEAT 93 133 WD 2.
 REPEAT 135 175 WD 3.
 MOD_RES 640 640 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; Autophagy; Complete proteome; Cytoplasmic vesicle; Developmental protein; Differentiation; Neurogenesis; Phosphoprotein; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1300 AA 
Protein Sequence
MKVVPEKNAV RILWGRERGT RAMGAQRLLQ ELVEDKTRWM KWEGKRVELP DSPRSTFLLA 60
FSPDRTLLAS THVNHNIYIT EVKTGKCVHS LIGHRRTPWC VTFHPTISGL IASGCLDGEV 120
RIWDLHGGSE SWFTDSNNAI ASLAFHPTAQ LLLIATANEI HFWDWSRREP FAVVKTASEM 180
ERVRLVRFDP LGHYLLTAIV NPSNQQGDDE PEIPIDGTEL SHYRQRALLQ SQPVRRTPLL 240
HNFLHMLSSR SSGIQVGEQS TVQDSATPSP PPPPPQPSTE RPRTSAYIRL RQRVSYPTTV 300
ECCQHPGILC LCSRCAGTRV PSLLPHQDSV PPASARATTP SFSFVQTEPF HPPEQASSTQ 360
QDQGLLNRPS AFSTVQSSTA GNTLRNLSLG PTRRSLGGPL SSHPSRYHRE LAPGLTGSEW 420
TRTVLTLNSR SEVESMPPPR TSASSVSLLS VLRQQEGGSQ ASVYTSATEG RGFPSSGLAT 480
ESDGGNGSSQ NNSGSIRHEL QCDLRRFFLE YDRLQELDQS LSGETPQTQQ AQEMLNNNIE 540
SERPGPSHLP TPHSSENNSN LSRGHLNRCR ACHNLLTFNN DTLRWERTTP NYSSGEASSS 600
WHVSTTFEGM PPSGNQLPPL ERTEGQMPSS SRLELSSSAS SQEERTVGVA FNQETGHWER 660
IYTQSSRSGT VSQEALHQDM PEESSEEDSL RRRLLESSLI SLSRYDGAGS REHPIYPDPA 720
RLSPAAYYAQ RMIQYLSRRD SIRQRSMRYQ QNRLRSSTSS SSSDNQGPSV EGTDLEFEDF 780
EDNGDRSRHR APRNARMSAP SLGRFVPRRF LLPEYLPYAG IFHERGQPGL ATHSSVNRVL 840
AGAVIGDGQS AVASNIANTT YRLQWWDFTK FDLPEISNAS VNVLVQNCKI YNDASCDISA 900
DGQLLAAFIP SSQRGFPDEG ILAVYSLAPH NLGEMLYTKR FGPNAISVSL SPMGRYVMVG 960
LASRRILLHP STEHMVAQVF RLQQAHGGET SMRRVFNVLY PMPADQRRHV SINSARWLPE 1020
PGLGLAYGTN KGDLVICRPE ALNSGIEYYW DQLSETVFTV HSSSRSSERP GTSRATWRTD 1080
RDMGLMNAIG LQPRNPTTSV TSQGTQTLAL QLQNAETQTE REEEEPGAAS SGPGEGEGSE 1140
YGGSGEDALS RIQRLMAEGG MTAVVQREQS TTMASMGGFG NNIIVSHRIH RSSQTGTESG 1200
AARTSSPQPS TSRGLPSEPG QLAERALSPR TASWDQPSTS GRELPQPALS SSSPVPIPVP 1260
LASNEGPTMH CNVTNNSHLP EGDGSNRGEA AGPSGEPQNR 1300 
Gene Ontology
 GO:0005776; C:autophagic vacuole; IEA:UniProtKB-SubCell.
 GO:0005737; C:cytoplasm; ISA:MGI.
 GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
 GO:0006914; P:autophagy; IMP:MGI.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0008285; P:negative regulation of cell proliferation; IMP:MGI.
 GO:0021915; P:neural tube development; IMP:MGI.
 GO:0043065; P:positive regulation of apoptotic process; IMP:MGI. 
Interpro
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS