CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037378
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Peroxisome biogenesis factor 1 
Protein Synonyms/Alias
  
Gene Name
 PEX1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
133QPRENLPKDISEEDIubiquitination[1]
189VHSWEKEKDKNIFLLubiquitination[1]
191SWEKEKDKNIFLLSPubiquitination[1]
434LCNVIKNKLDCDINKubiquitination[1]
441KLDCDINKFTDLDLQubiquitination[1]
484QSISTREKLVLTTLDubiquitination[2, 3]
494LTTLDFQKALRGFLPubiquitination[1, 3]
511LRSVNLHKPRDLGWDubiquitination[1]
519PRDLGWDKIGGLHEVubiquitination[1]
565YGPPGTGKTLLAGVIubiquitination[1]
584RMNFISVKGPELLSKubiquitination[1]
591KGPELLSKYIGASEQubiquitination[1, 4]
611FIRAQAAKPCILFFDubiquitination[1]
679LRPGRLDKCVYCPPPubiquitination[1]
882RADISIIKGRYRSQSubiquitination[1]
902MNQPGPIKTRLAISQubiquitination[1, 5]
942YESFQNPKRRKNQSGubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 961 AA 
Protein Sequence
MWGSDRLAGA GGGGAAVTVA FTNARDCFLH LPRRLVAQLH LLQNQAIEVV WSHQPAFLSW 60
VEGRHFSDQG ENVAEINRQV GQKLGLSNGG QIPDDLRKRL NIEMHAVVRI TPVEVTPKIP 120
RSLKLQPREN LPKDISEEDI KTVFYSWLQQ STTTMLPLVI SEEEFIKLET KDGLKEFSLS 180
IVHSWEKEKD KNIFLLSPNL LQKTTIQVLL DPMVKEENSE EIDFILPFLK LSSLGGVNSL 240
GVSSLEHITH SLLGRPLSRQ LMSLVAGLRN GALLLTGGKG SGKSTLAKAI CKEAFDKLDA 300
HVERVDCKAL RGKRLENIQK TLEVAFSEAV WMQPSVVLLD DLDLIAGLPA VPEHEHSPDA 360
VQSQRLAHAL NDMIKEFISM GSLVALIATS QSQQSLHPLL VSAQGVHIFQ CVQHIQPPNQ 420
EQRCEILCNV IKNKLDCDIN KFTDLDLQHV AKETGGFVAR DFTVLVDRAI HSRLSRQSIS 480
TREKLVLTTL DFQKALRGFL PASLRSVNLH KPRDLGWDKI GGLHEVRQIL MDTIQLPAKY 540
PELFANLPIR QRTGILLYGP PGTGKTLLAG VIARESRMNF ISVKGPELLS KYIGASEQAV 600
RDIFIRAQAA KPCILFFDEF ESIAPRRGHD NTGVTDRVVN QLLTQLDGVE GLQGVYVLAA 660
TSRPDLIDPA LLRPGRLDKC VYCPPPDQVS RLEILNVLSD SLPLADDVDL QHVASVTDSF 720
TGADLKALLY NAQLEALHGM LLSSGLQDGS SSSDSDLSLS SMVFLNHSSG SDDSAGDGEC 780
GLDQSLVSLE MSEILPDESK FNMYRLYFGS SYESELGNGT SSDLSSQCLS APSSMTQDLP 840
GVPGKDQLFS QPPVLRTASQ EGCQELTQEQ RDQLRADISI IKGRYRSQSG EDESMNQPGP 900
IKTRLAISQS HLMTALGHTR PSISEDDWKN FAELYESFQN PKRRKNQSGT MFRPGQKVTL 960
A 961 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005778; C:peroxisomal membrane; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0042623; F:ATPase activity, coupled; IEA:InterPro.
 GO:0006625; P:protein targeting to peroxisome; IEA:InterPro. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR009010; Asp_de-COase-like_dom.
 IPR003959; ATPase_AAA_core.
 IPR003960; ATPase_AAA_CS.
 IPR027417; P-loop_NTPase.
 IPR015343; Peroxisome_synth_fac_1_a/b.
 IPR025653; Pex1. 
Pfam
 PF00004; AAA
 PF09263; PEX-2N 
SMART
 SM00382; AAA 
PROSITE
 PS00674; AAA 
PRINTS