CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003347
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Hydrogenase-2 large chain 
Protein Synonyms/Alias
 HYD2; Membrane-bound hydrogenase 2 large subunit; NiFe hydrogenase 
Gene Name
 hybC 
Gene Synonyms/Alias
 b2994; JW2962 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
135TKASEMLKGVSTWHLacetylation[1]
150NSPEEFTKVQNKIKDacetylation[1]
366DGKYSWVKSPTFYGKacetylation[1]
460GDHTTFVKPNIPATGacetylation[1]
470IPATGEFKGVGFLEAacetylation[1]
532TPVADPNKPLEVVRTacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake. 
Sequence Annotation
 METAL 61 61 Nickel (Potential).
 METAL 64 64 Nickel (Potential).
 METAL 546 546 Nickel (Potential).
 METAL 549 549 Nickel (Potential).  
Keyword
 Cell membrane; Complete proteome; Direct protein sequencing; Membrane; Metal-binding; Nickel; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 567 AA 
Protein Sequence
MSQRITIDPV TRIEGHLRID CEIENGVVSK AWASGTMWRG MEEIVKNRDP RDAWMIVQRI 60
CGVCTTTHAL SSVRAAESAL NIDVPVNAQY IRNIILAAHT THDHIVHFYQ LSALDWVDIT 120
SALQADPTKA SEMLKGVSTW HLNSPEEFTK VQNKIKDLVA SGQLGIFANG YWGHPAMKLP 180
PEVNLIAVAH YLQALECQRD ANRVVALLGG KTPHIQNLAV GGVANPINLD GLGVLNLERL 240
MYIKSFIDKL SDFVEQVYKV DTAVIAAFYP EWLTRGKGAV NYLSVPEFPT DSKNGSFLFP 300
GGYIENADLS SYRPITSHSD EYLIKGIQES AKHSWYKDEA PQAPWEGTTI PAYDGWSDDG 360
KYSWVKSPTF YGKTVEVGPL ANMLVKLAAG RESTQNKLNE IVAIYQKLTG NTLEVAQLHS 420
TLGRIIGRTV HCCELQDILQ NQYSALITNI GKGDHTTFVK PNIPATGEFK GVGFLEAPRG 480
MLSHWMVIKD GIISNYQAVV PSTWNSGPRN FNDDVGPYEQ SLVGTPVADP NKPLEVVRTI 540
HSFDPCMACA VHVVDADGNE VVSVKVL 567 
Gene Ontology
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
 GO:0033748; F:hydrogenase (acceptor) activity; IEA:EC.
 GO:0016151; F:nickel cation binding; IEA:InterPro. 
Interpro
 IPR001501; Ni-dep_hyd_lsu.
 IPR018194; Ni-dep_hyd_lsu_Ni_BS. 
Pfam
 PF00374; NiFeSe_Hases 
SMART
  
PROSITE
 PS00507; NI_HGENASE_L_1
 PS00508; NI_HGENASE_L_2 
PRINTS