CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013199
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glutamine synthetase, chloroplastic/mitochondrial 
Protein Synonyms/Alias
 GS2; Glutamate--ammonia ligase 
Gene Name
 GLN2 
Gene Synonyms/Alias
 GSLI; At5g35630; MJE4.9 
Created Date
 July 27, 2013 
Organism
 Arabidopsis thaliana (Mouse-ear cress) 
NCBI Taxa ID
 3702 
Lysine Modification
Position
Peptide
Type
References
223YCGVGADKIWGRDISacetylation[1]
Reference
 [1] Proteins of diverse function and subcellular location are lysine acetylated in Arabidopsis.
 Finkemeier I, Laxa M, Miguet L, Howden AJ, Sweetlove LJ.
 Plant Physiol. 2011 Apr;155(4):1779-90. [PMID: 21311031] 
Functional Description
 The light-modulated chloroplast/mitochondrial enzyme, encoded by a nuclear gene and expressed primarily in leaves, is responsible for the reassimilation of the ammonia generated by photorespiration. 
Sequence Annotation
 MOD_RES 106 106 Phosphoserine.  
Keyword
 ATP-binding; Chloroplast; Complete proteome; Ligase; Mitochondrion; Nucleotide-binding; Phosphoprotein; Plastid; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 430 AA 
Protein Sequence
MAQILAASPT CQMRVPKHSS VIASSSKLWS SVVLKQKKQS NNKVRGFRVL ALQSDNSTVN 60
RVETLLNLDT KPYSDRIIAE YIWIGGSGID LRSKSRTIEK PVEDPSELPK WNYDGSSTGQ 120
APGEDSEVIL YPQAIFRDPF RGGNNILVIC DTWTPAGEPI PTNKRAKAAE IFSNKKVSGE 180
VPWFGIEQEY TLLQQNVKWP LGWPVGAFPG PQGPYYCGVG ADKIWGRDIS DAHYKACLYA 240
GINISGTNGE VMPGQWEFQV GPSVGIDAGD HVWCARYLLE RITEQAGVVL TLDPKPIEGD 300
WNGAGCHTNY STKSMREEGG FEVIKKAILN LSLRHKEHIS AYGEGNERRL TGKHETASID 360
QFSWGVANRG CSIRVGRDTE AKGKGYLEDR RPASNMDPYI VTSLLAETTL LWEPTLEAEA 420
LAAQKLSLNV 430 
Gene Ontology
 GO:0048046; C:apoplast; IDA:TAIR.
 GO:0009941; C:chloroplast envelope; IDA:TAIR.
 GO:0009570; C:chloroplast stroma; IDA:TAIR.
 GO:0009535; C:chloroplast thylakoid membrane; IDA:TAIR.
 GO:0022626; C:cytosolic ribosome; IDA:TAIR.
 GO:0005739; C:mitochondrion; IDA:TAIR.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004356; F:glutamate-ammonia ligase activity; IDA:TAIR.
 GO:0007568; P:aging; TAS:TAIR.
 GO:0019676; P:ammonia assimilation cycle; TAS:TAIR.
 GO:0006542; P:glutamine biosynthetic process; IEA:InterPro.
 GO:0046686; P:response to cadmium ion; IEP:TAIR.
 GO:0009651; P:response to salt stress; IEP:TAIR. 
Interpro
 IPR008147; Gln_synt_beta.
 IPR014746; Gln_synth/guanido_kin_cat_dom.
 IPR008146; Gln_synth_cat_dom.
 IPR027303; Gln_synth_gly_rich_site.
 IPR027302; Gln_synth_N_conserv_site. 
Pfam
 PF00120; Gln-synt_C
 PF03951; Gln-synt_N 
SMART
  
PROSITE
 PS00180; GLNA_1
 PS00181; GLNA_ATP 
PRINTS