CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007548
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Signal transducer and activator of transcription 5A 
Protein Synonyms/Alias
  
Gene Name
 STAT5A 
Gene Synonyms/Alias
 STAT5 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
71LVQELQKKAEHQVGEubiquitination[1]
84GEDGFLLKIKLGHYAacetylation[2]
163QDTENELKKLQQTQEubiquitination[3]
164DTENELKKLQQTQEYubiquitination[1]
207RETALQQKQVSLEAWubiquitination[1, 3, 4]
235ELAEKHQKTLQLLRKubiquitination[1]
242KTLQLLRKQQTIILDubiquitination[1]
516EALNMKFKAEVQSNRubiquitination[1]
527QSNRGLTKENLVFLAubiquitination[1, 5, 6]
644ERNLWNLKPFTTRDFubiquitination[1, 3]
675YVFPDRPKDEVFSKYubiquitination[1]
689YYTPVLAKAVDGYVKubiquitination[1, 3]
696KAVDGYVKPQIKQVVacetylation[7]
696KAVDGYVKPQIKQVVsumoylation[8]
696KAVDGYVKPQIKQVVubiquitination[1, 3]
700GYVKPQIKQVVPEFVsumoylation[8]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [8] SUMO-specific protease 1 is critical for early lymphoid development through regulation of STAT5 activation.
 Van Nguyen T, Angkasekwinai P, Dou H, Lin FM, Lu LS, Cheng J, Chin YE, Dong C, Yeh ET.
 Mol Cell. 2012 Jan 27;45(2):210-21. [PMID: 22284677] 
Functional Description
 Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation. 
Sequence Annotation
 DOMAIN 589 686 SH2.
 MOD_RES 128 128 Phosphoserine (By similarity).
 MOD_RES 193 193 Phosphoserine.
 MOD_RES 694 694 Phosphotyrosine; by JAK2.  
Keyword
 Activator; Complete proteome; Cytoplasm; DNA-binding; Lactation; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; SH2 domain; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 794 AA 
Protein Sequence
MAGWIQAQQL QGDALRQMQV LYGQHFPIEV RHYLAQWIES QPWDAIDLDN PQDRAQATQL 60
LEGLVQELQK KAEHQVGEDG FLLKIKLGHY ATQLQKTYDR CPLELVRCIR HILYNEQRLV 120
REANNCSSPA GILVDAMSQK HLQINQTFEE LRLVTQDTEN ELKKLQQTQE YFIIQYQESL 180
RIQAQFAQLA QLSPQERLSR ETALQQKQVS LEAWLQREAQ TLQQYRVELA EKHQKTLQLL 240
RKQQTIILDD ELIQWKRRQQ LAGNGGPPEG SLDVLQSWCE KLAEIIWQNR QQIRRAEHLC 300
QQLPIPGPVE EMLAEVNATI TDIISALVTS TFIIEKQPPQ VLKTQTKFAA TVRLLVGGKL 360
NVHMNPPQVK ATIISEQQAK SLLKNENTRN ECSGEILNNC CVMEYHQATG TLSAHFRNMS 420
LKRIKRADRR GAESVTEEKF TVLFESQFSV GSNELVFQVK TLSLPVVVIV HGSQDHNATA 480
TVLWDNAFAE PGRVPFAVPD KVLWPQLCEA LNMKFKAEVQ SNRGLTKENL VFLAQKLFNN 540
SSSHLEDYSG LSVSWSQFNR ENLPGWNYTF WQWFDGVMEV LKKHHKPHWN DGAILGFVNK 600
QQAHDLLINK PDGTFLLRFS DSEIGGITIA WKFDSPERNL WNLKPFTTRD FSIRSLADRL 660
GDLSYLIYVF PDRPKDEVFS KYYTPVLAKA VDGYVKPQIK QVVPEFVNAS ADAGGSSATY 720
MDQAPSPAVC PQAPYNMYPQ NPDHVLDQDG EFDLDETMDV ARHVEELLRR PMDSLDSRLS 780
PPAGLFTSAR GSLS 794 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0000979; F:RNA polymerase II core promoter sequence-specific DNA binding; IEA:Compara.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:ProtInc.
 GO:0004871; F:signal transducer activity; IEA:Compara.
 GO:0006103; P:2-oxoglutarate metabolic process; ISS:BHF-UCL.
 GO:0000255; P:allantoin metabolic process; ISS:BHF-UCL.
 GO:0006101; P:citrate metabolic process; ISS:BHF-UCL.
 GO:0006600; P:creatine metabolic process; ISS:BHF-UCL.
 GO:0046449; P:creatinine metabolic process; ISS:BHF-UCL.
 GO:0019221; P:cytokine-mediated signaling pathway; IEA:Compara.
 GO:0046543; P:development of secondary female sexual characteristics; IEA:Compara.
 GO:0046544; P:development of secondary male sexual characteristics; IEA:Compara.
 GO:0060742; P:epithelial cell differentiation involved in prostate gland development; IEA:Compara.
 GO:0006631; P:fatty acid metabolic process; ISS:BHF-UCL.
 GO:0007565; P:female pregnancy; IEA:Compara.
 GO:0006549; P:isoleucine metabolic process; ISS:BHF-UCL.
 GO:0060397; P:JAK-STAT cascade involved in growth hormone signaling pathway; TAS:Reactome.
 GO:0007595; P:lactation; IEA:UniProtKB-KW.
 GO:0019915; P:lipid storage; IEA:Compara.
 GO:0001553; P:luteinization; IEA:Compara.
 GO:0061180; P:mammary gland epithelium development; IEA:Compara.
 GO:0001779; P:natural killer cell differentiation; IEA:Compara.
 GO:0045647; P:negative regulation of erythrocyte differentiation; IEA:Compara.
 GO:0033026; P:negative regulation of mast cell apoptotic process; IEA:Compara.
 GO:0006107; P:oxaloacetate metabolic process; ISS:BHF-UCL.
 GO:0018108; P:peptidyl-tyrosine phosphorylation; IEA:Compara.
 GO:0048541; P:Peyer's patch development; IEA:Compara.
 GO:0042104; P:positive regulation of activated T cell proliferation; IEA:Compara.
 GO:0045579; P:positive regulation of B cell differentiation; IEA:Compara.
 GO:0045588; P:positive regulation of gamma-delta T cell differentiation; IEA:Compara.
 GO:0050729; P:positive regulation of inflammatory response; IEA:Compara.
 GO:0045086; P:positive regulation of interleukin-2 biosynthetic process; IEA:Compara.
 GO:0060376; P:positive regulation of mast cell differentiation; IEA:Compara.
 GO:0070668; P:positive regulation of mast cell proliferation; IEA:Compara.
 GO:0045931; P:positive regulation of mitotic cell cycle; IEA:Compara.
 GO:0040018; P:positive regulation of multicellular organism growth; IEA:Compara.
 GO:0032825; P:positive regulation of natural killer cell differentiation; IEA:Compara.
 GO:0045954; P:positive regulation of natural killer cell mediated cytotoxicity; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0060740; P:prostate gland epithelium morphogenesis; IEA:Compara.
 GO:0030155; P:regulation of cell adhesion; IEA:Compara.
 GO:0030856; P:regulation of epithelial cell differentiation; IEA:Compara.
 GO:0040014; P:regulation of multicellular organism growth; ISS:BHF-UCL.
 GO:0019218; P:regulation of steroid metabolic process; IEA:Compara.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; TAS:ProtInc.
 GO:0006105; P:succinate metabolic process; ISS:BHF-UCL.
 GO:0033077; P:T cell differentiation in thymus; IEA:Compara.
 GO:0043029; P:T cell homeostasis; IEA:Compara.
 GO:0019530; P:taurine metabolic process; ISS:BHF-UCL.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006573; P:valine metabolic process; ISS:BHF-UCL. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR008967; p53-like_TF_DNA-bd.
 IPR000980; SH2.
 IPR013800; STAT_TF_alpha.
 IPR015988; STAT_TF_coiled-coil.
 IPR001217; STAT_TF_core.
 IPR013801; STAT_TF_DNA-bd.
 IPR012345; STAT_TF_DNA-bd_sub.
 IPR013799; STAT_TF_prot_interaction. 
Pfam
 PF00017; SH2
 PF01017; STAT_alpha
 PF02864; STAT_bind
 PF02865; STAT_int 
SMART
 SM00252; SH2
 SM00964; STAT_int 
PROSITE
 PS50001; SH2 
PRINTS