CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023889
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 YTH domain family protein 2 
Protein Synonyms/Alias
 CLL-associated antigen KW-14; High-glucose-regulated protein 8; Renal carcinoma antigen NY-REN-2 
Gene Name
 YTHDF2 
Gene Synonyms/Alias
 HGRG8 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
12SLLEQRPKGQGNKVQubiquitination[1, 2, 3]
17RPKGQGNKVQNGSVHubiquitination[3]
26QNGSVHQKDGLNDDDubiquitination[2, 3]
193DQGMAALKLGSTEVAubiquitination[2, 3, 4, 5]
245SWADIASKPAKQQPKacetylation[6, 7, 8]
281DIGTWDNKGPVAKAPubiquitination[3, 4]
391EPHPVLEKLRSINNYubiquitination[9]
401SINNYNPKDFDWNLKubiquitination[2, 10, 11]
408KDFDWNLKHGRVFIIubiquitination[2, 3, 10, 11]
416HGRVFIIKSYSEDDIubiquitination[4]
492VWSQDKWKGRFDVRWubiquitination[2]
521RLENNENKPVTNSRDubiquitination[2, 3]
562DDFSHYEKRQEEEESubiquitination[2, 3]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [7] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [8] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [9] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [10] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [11] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
  
Sequence Annotation
 DOMAIN 410 544 YTH.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 39 39 Phosphoserine.
 MOD_RES 359 359 Phosphoserine.
 MOD_RES 394 394 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 579 AA 
Protein Sequence
MSASSLLEQR PKGQGNKVQN GSVHQKDGLN DDDFEPYLSP QARPNNAYTA MSDSYLPSYY 60
SPSIGFSYSL GEAAWSTGGD TAMPYLTSYG QLSNGEPHFL PDAMFGQPGA LGSTPFLGQH 120
GFNFFPSGID FSAWGNNSSQ GQSTQSSGYS SNYAYAPSSL GGAMIDGQSA FANETLNKAP 180
GMNTIDQGMA ALKLGSTEVA SNVPKVVGSA VGSGSITSNI VASNSLPPAT IAPPKPASWA 240
DIASKPAKQQ PKLKTKNGIA GSSLPPPPIK HNMDIGTWDN KGPVAKAPSQ ALVQNIGQPT 300
QGSPQPVGQQ ANNSPPVAQA SVGQQTQPLP PPPPQPAQLS VQQQAAQPTR WVAPRNRGSG 360
FGHNGVDGNG VGQSQAGSGS TPSEPHPVLE KLRSINNYNP KDFDWNLKHG RVFIIKSYSE 420
DDIHRSIKYN IWCSTEHGNK RLDAAYRSMN GKGPVYLLFS VNGSGHFCGV AEMKSAVDYN 480
TCAGVWSQDK WKGRFDVRWI FVKDVPNSQL RHIRLENNEN KPVTNSRDTQ EVPLEKAKQV 540
LKIIASYKHT TSIFDDFSHY EKRQEEEESV KKERQGRGK 579 
Gene Ontology
 GO:0006959; P:humoral immune response; TAS:ProtInc. 
Interpro
 IPR007275; YTH_domain. 
Pfam
 PF04146; YTH 
SMART
  
PROSITE
 PS50882; YTH 
PRINTS