CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006452
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Merlin 
Protein Synonyms/Alias
 Moesin-ezrin-radixin-like protein; Neurofibromin-2; Schwannomerlin; Schwannomin 
Gene Name
 NF2 
Gene Synonyms/Alias
 SCH 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
159DYDPSVHKRGFLAQEubiquitination[1]
269RNISYSDKEFTIKPLubiquitination[2]
364ERRLLQMKEEATMANubiquitination[3]
387TADLLAEKAQITEEEubiquitination[1]
396QITEEEAKLLAQKAAubiquitination[1, 2, 3]
533VEYMEKSKHLQEQLNubiquitination[1, 2, 3]
573SDRGGSSKHNTIKKSubiquitination[3]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 
Functional Description
 Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex. 
Sequence Annotation
 DOMAIN 22 311 FERM.
 MOD_RES 518 518 Phosphoserine; by PAK.  
Keyword
 3D-structure; Alternative splicing; Cell membrane; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; Deafness; Disease mutation; Membrane; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Tumor suppressor; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 595 AA 
Protein Sequence
MAGAIASRMS FSSLKRKQPK TFTVRIVTMD AEMEFNCEMK WKGKDLFDLV CRTLGLRETW 60
FFGLQYTIKD TVAWLKMDKK VLDHDVSKEE PVTFHFLAKF YPENAEEELV QEITQHLFFL 120
QVKKQILDEK IYCPPEASVL LASYAVQAKY GDYDPSVHKR GFLAQEELLP KRVINLYQMT 180
PEMWEERITA WYAEHRGRAR DEAEMEYLKI AQDLEMYGVN YFAIRNKKGT ELLLGVDALG 240
LHIYDPENRL TPKISFPWNE IRNISYSDKE FTIKPLDKKI DVFKFNSSKL RVNKLILQLC 300
IGNHDLFMRR RKADSLEVQQ MKAQAREEKA RKQMERQRLA REKQMREEAE RTRDELERRL 360
LQMKEEATMA NEALMRSEET ADLLAEKAQI TEEEAKLLAQ KAAEAEQEMQ RIKATAIRTE 420
EEKRLMEQKV LEAEVLALKM AEESERRAKE ADQLKQDLQE AREAERRAKQ KLLEIATKPT 480
YPPMNPIPAP LPPDIPSFNL IGDSLSFDFK DTDMKRLSME IEKEKVEYME KSKHLQEQLN 540
ELKTEIEALK LKERETALDI LHNENSDRGG SSKHNTIKKS SPRQKTYLHL SPQSRLFPGT 600
LYVVMLYVVM VLPSVILTRA 620 
Gene Ontology
 GO:0005912; C:adherens junction; IEA:Compara.
 GO:0032154; C:cleavage furrow; IEA:Compara.
 GO:0030864; C:cortical actin cytoskeleton; IEA:Compara.
 GO:0005856; C:cytoskeleton; TAS:ProtInc.
 GO:0005769; C:early endosome; IDA:HGNC.
 GO:0019898; C:extrinsic to membrane; IEA:InterPro.
 GO:0031527; C:filopodium membrane; IEA:UniProtKB-SubCell.
 GO:0030027; C:lamellipodium; IEA:Compara.
 GO:0005730; C:nucleolus; IDA:HGNC.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:HGNC.
 GO:0005886; C:plasma membrane; IDA:UniProtKB.
 GO:0032587; C:ruffle membrane; IEA:UniProtKB-SubCell.
 GO:0030036; P:actin cytoskeleton organization; IMP:HGNC.
 GO:0045216; P:cell-cell junction organization; IEA:Compara.
 GO:0007398; P:ectoderm development; IEA:Compara.
 GO:0070306; P:lens fiber cell differentiation; IEA:Compara.
 GO:0001707; P:mesoderm formation; IEA:Compara.
 GO:0030336; P:negative regulation of cell migration; TAS:HGNC.
 GO:0008285; P:negative regulation of cell proliferation; IDA:UniProtKB.
 GO:0022408; P:negative regulation of cell-cell adhesion; IDA:HGNC.
 GO:0001953; P:negative regulation of cell-matrix adhesion; TAS:HGNC.
 GO:0008156; P:negative regulation of DNA replication; IMP:HGNC.
 GO:0043409; P:negative regulation of MAPK cascade; IEA:Compara.
 GO:0006469; P:negative regulation of protein kinase activity; IEA:Compara.
 GO:0042518; P:negative regulation of tyrosine phosphorylation of Stat3 protein; IDA:HGNC.
 GO:0042524; P:negative regulation of tyrosine phosphorylation of Stat5 protein; IDA:HGNC.
 GO:0042475; P:odontogenesis of dentin-containing tooth; IEA:Compara.
 GO:0051496; P:positive regulation of stress fiber assembly; IMP:HGNC.
 GO:0035330; P:regulation of hippo signaling cascade; IMP:UniProtKB.
 GO:0014010; P:Schwann cell proliferation; IMP:HGNC. 
Interpro
 IPR019749; Band_41_domain.
 IPR019750; Band_41_fam.
 IPR011174; ERM.
 IPR011259; ERM_C_dom.
 IPR000798; Ez/rad/moesin_like.
 IPR014352; FERM/acyl-CoA-bd_prot_3-hlx.
 IPR019748; FERM_central.
 IPR019747; FERM_CS.
 IPR000299; FERM_domain.
 IPR018979; FERM_N.
 IPR018980; FERM_PH-like_C.
 IPR008954; Moesin.
 IPR011993; PH_like_dom. 
Pfam
 PF00769; ERM
 PF09380; FERM_C
 PF00373; FERM_M
 PF09379; FERM_N 
SMART
 SM00295; B41 
PROSITE
 PS00660; FERM_1
 PS00661; FERM_2
 PS50057; FERM_3 
PRINTS
 PR00935; BAND41.
 PR00661; ERMFAMILY.