UniProt Accession

 UBC7_YEAST; Q02159; D6VZJ6 

Genbank Protein ID

 X66829; X69100; Z49211; AY558116; BK006946 

Genbank Nucleotide ID

 CAA47302.1; CAA48846.1; CAA89125.1; AAS56442.1; DAA09920.1 

Protein Name

 Ubiquitin-conjugating enzyme E2 7 

Protein Synonyms/Alias

 Ubiquitin carrier protein; Ubiquitin-conjugating enzyme E2-18 kDa; Ubiquitin-protein ligase 

Gene Name

 UBC7 

Gene Synonyms/Alias

 QRI8; YMR022W; YM9711.12 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
62	NAKLEFPKDYPLSPP	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in degradation of misfolded or regulated proteins localized in the endoplasmic reticulum (ER) lumen or membrane via the ubiquitin-proteasome system. Cognate E2 conjugating enzyme for the DOA10 ubiquitin ligase complex, which is part of the ERAD-C pathway responsible for the rapid degradation of membrane proteins with misfolded cytoplasmic domains, and of the HRD1 ubiquitin ligase complex, which is part of the ERAD-L and ERAD-M pathways responsible for the rapid degradation of soluble lumenal and membrane proteins with misfolded lumenal domains (ERAD-L), or ER-membrane proteins with misfolded transmembrane domains (ERAD-M). Involved in resistance to cadmium poisoning. 

Sequence Annotation

 ACT_SITE 89 89 Glycyl thioester intermediate.
 CROSSLNK 89 89 Glycyl cysteine thioester (Cys-Gly)  

Keyword

 3D-structure; ATP-binding; Cadmium; Cadmium resistance; Complete proteome; Endoplasmic reticulum; Ligase; Membrane; Nucleotide-binding; Reference proteome; Thioester bond; Ubl conjugation; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 165 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004842; F:ubiquitin-protein ligase activity; IDA:SGD.
 GO:0006333; P:chromatin assembly or disassembly; IMP:SGD.
 GO:0030433; P:ER-associated protein catabolic process; IDA:SGD.
 GO:0031505; P:fungal-type cell wall organization; IGI:SGD.
 GO:0046686; P:response to cadmium ion; IEA:UniProtKB-KW. 

Interpro

 IPR000608; UBQ-conjugat_E2.
 IPR023313; UBQ-conjugating_AS.
 IPR016135; UBQ-conjugating_enzyme/RWD. 

Pfam

 PF00179; UQ_con 

SMART

  

PROSITE

 PS00183; UBIQUITIN_CONJUGAT_1
 PS50127; UBIQUITIN_CONJUGAT_2 

PRINTS