UniProt Accession

 ITPK1_HUMAN; Q13572; Q9BTL6; Q9H2E7 

Genbank Protein ID

 U51336; AF279372; BC003622; BC007428; BC018192 

Genbank Nucleotide ID

 AAC50483.1; AAG44835.1; AAH03622.1; AAH07428.1; AAH18192.1 

Protein Name

 Inositol-tetrakisphosphate 1-kinase 

Protein Synonyms/Alias

 Inositol 1,3,4-trisphosphate 5/6-kinase; Inositol-triphosphate 5/6-kinase; Ins(1,3,4)P(3) 5/6-kinase 

Gene Name

 ITPK1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
147	DTMRLLEKNGLTFPF	ubiquitination	[1]
340	VALLRHSKLLAEPAG	acetylation	[2]
340	VALLRHSKLLAEPAG	ubiquitination	[1]
383	ADAGGTAKLPHQRLG	acetylation	[2]
410	CVASLATKASSQ***	acetylation	[2]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Regulation of inositol 1,3,4-trisphosphate 5/6-kinase (ITPK1) by reversible lysine acetylation.
 Zhang C, Majerus PW, Wilson MP.
 Proc Natl Acad Sci U S A. 2012 Feb 14;109(7):2290-5. [PMID: 22308441] 

Functional Description

 Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3. Phosphorylates Ins(3,4,5,6)P4 at position 1 to form Ins(1,3,4,5,6)P5. This reaction is thought to have regulatory importance, since Ins(3,4,5,6)P4 is an inhibitor of plasma membrane Ca(2+)-activated Cl(-) channels, while Ins(1,3,4,5,6)P5 is not. Also phosphorylates Ins(1,3,4)P3 on O-5 and O-6 to form Ins(1,3,4,6)P4, an essential molecule in the hexakisphosphate (InsP6) pathway. Also acts as an inositol polyphosphate phosphatase that dephosphorylate Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 to Ins(1,3,4)P3, and Ins(1,3,4,5,6)P5 to Ins(3,4,5,6)P4. May also act as an isomerase that interconverts the inositol tetrakisphosphate isomers Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP and magnesium. Probably acts as the rate-limiting enzyme of the InsP6 pathway. Modifies TNF-alpha-induced apoptosis by interfering with the activation of TNFRSF1A-associated death domain. 

Sequence Annotation

 DOMAIN 117 325 ATP-grasp.
 NP_BIND 188 199 ATP.
 METAL 281 281 Magnesium 1.
 METAL 295 295 Magnesium 1.
 METAL 295 295 Magnesium 2.
 METAL 297 297 Magnesium 2.
 BINDING 18 18 1D-myo-inositol 1,3,4-trisphosphate (By
 BINDING 106 106 ATP.
 BINDING 157 157 ATP.
 BINDING 167 167 1D-myo-inositol 1,3,4-trisphosphate (By
 BINDING 199 199 1D-myo-inositol 1,3,4-trisphosphate (By
 BINDING 214 214 ATP.
 BINDING 232 232 ATP.
 BINDING 236 236 ATP.
 BINDING 297 297 1D-myo-inositol 1,3,4-trisphosphate (By
 MOD_RES 340 340 N6-acetyllysine; by EP300 and CREBBP.
 MOD_RES 383 383 N6-acetyllysine; by EP300 and CREBBP.
 MOD_RES 410 410 N6-acetyllysine; by EP300 and CREBBP.  

Keyword

 3D-structure; Acetylation; Alternative splicing; ATP-binding; Complete proteome; Hydrolase; Isomerase; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 414 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0047325; F:inositol tetrakisphosphate 1-kinase activity; TAS:Reactome.
 GO:0052825; F:inositol-1,3,4,5,6-pentakisphosphate 1-phosphatase activity; TAS:Reactome.
 GO:0052831; F:inositol-1,3,4,6-tetrakisphosphate 1-phosphatase activity; TAS:Reactome.
 GO:0052830; F:inositol-1,3,4,6-tetrakisphosphate 6-phosphatase activity; TAS:Reactome.
 GO:0052726; F:inositol-1,3,4-trisphosphate 5-kinase activity; TAS:Reactome.
 GO:0052725; F:inositol-1,3,4-trisphosphate 6-kinase activity; TAS:Reactome.
 GO:0052835; F:inositol-3,4,6-trisphosphate 1-kinase activity; TAS:Reactome.
 GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0043647; P:inositol phosphate metabolic process; TAS:Reactome.
 GO:0032957; P:inositol trisphosphate metabolic process; IEA:InterPro.
 GO:0007165; P:signal transduction; TAS:ProtInc. 

Interpro

 IPR011761; ATP-grasp.
 IPR008656; Inositol_tetrakis-P_1-kinase. 

Pfam

 PF05770; Ins134_P3_kin 

SMART

  

PROSITE

 PS50975; ATP_GRASP 

PRINTS