CPLM-018856

Genbank Nucleotide ID

CUGBP Elav-like family member 1

Protein Synonyms/Alias

CELF-1; 50 kDa nuclear polyadenylated RNA-binding protein; Bruno-like protein 2; CUG triplet repeat RNA-binding protein 1; CUG-BP1; CUG-BP- and ETR-3-like factor 1; Deadenylation factor CUG-BP; Embryo deadenylation element-binding protein homolog; EDEN-BP homolog; RNA-binding protein BRUNOL-2

BRUNOL2; CUGBP; CUGBP1; NAB50

Homo sapiens (Human)

Position	Peptide	Type	References
59	SQNPPQSKGCCFVTF	ubiquitination	[1]
70	FVTFYTRKAALEAQN	ubiquitination	[1]
118	FIGMISKKCTENDIR	ubiquitination	[2]
436	SAKVFIDKQTNLSKC	ubiquitination	[1]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]

Functional Description

RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre- mRNAs. Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB. Promotes exclusion of exon 11 of the INSR pre-mRNA. Inhibits, together with HNRNPH1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Increases translation and controls the choice of translation initiation codon of CEBPB mRNA. Increases mRNA translation of CEBPB in aging liver (By similarity). Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3. Mediates rapid cytoplasmic mRNA deadenylation. Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation. Required for completion of spermatogenesis (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK and to Bruno response elements (BREs). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3'-UTR of JUN and FOS mRNAs. Binds to the IR RNA. Binds to the 5'-region of CDKN1A and CEBPB mRNAs. Binds with the 5'-region of CEBPB mRNA in aging liver.

DOMAIN 16 99 RRM 1.
DOMAIN 108 188 RRM 2.
DOMAIN 401 479 RRM 3.
MOD_RES 1 1 N-acetylmethionine.
MOD_RES 302 302 Phosphoserine (By similarity).

3D-structure; Acetylation; Activator; Alternative splicing; Complete proteome; Cytoplasm; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; Repeat; RNA-binding.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO:0005634; C:nucleus; NAS:UniProtKB.
GO:0030529; C:ribonucleoprotein complex; NAS:UniProtKB.
GO:0042835; F:BRE binding; IDA:UniProtKB.
GO:0003729; F:mRNA binding; IDA:BHF-UCL.
GO:0000166; F:nucleotide binding; IEA:InterPro.
GO:0000900; F:translation repressor activity, nucleic acid binding; NAS:UniProtKB.
GO:0009790; P:embryo development; NAS:UniProtKB.
GO:0007281; P:germ cell development; NAS:UniProtKB.
GO:0006376; P:mRNA splice site selection; ISS:UniProtKB.
GO:0040018; P:positive regulation of multicellular organism growth; IEA:Compara.
GO:0043484; P:regulation of RNA splicing; IDA:UniProtKB.
GO:0016246; P:RNA interference; NAS:UniProtKB.
GO:0007286; P:spermatid development; IEA:Compara.

IPR012677; Nucleotide-bd_a/b_plait.
IPR000504; RRM_dom.