UniProt Accession

 YGHU_ECOLI; Q46845; Q2M9K6 

Genbank Protein ID

 U28377; U00096; AP009048 

Genbank Nucleotide ID

 AAA69156.1; AAC76025.2; BAE77050.1 

Protein Name

 Disulfide-bond oxidoreductase YghU 

Protein Synonyms/Alias

 GSH-dependent disulfide-bond oxidoreductase YghU; GST N2-2; Organic hydroperoxidase 

Gene Name

 yghU 

Gene Synonyms/Alias

 b2989; JW5492 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
16	AKVWTWDKSAGGAFA	acetylation	[1]
36	VSGPTHEKTLPVGKH	acetylation	[1]
42	EKTLPVGKHPLQLYS	acetylation	[1]
139	FLPQDLAKRTETMNW	acetylation	[1]
192	RLLDVLDKQLAQHKF	acetylation	[1]
244	KHVQRWAKEVGERPA	acetylation	[1]
285	FETNTEDKRQG****	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Exhibits a robust glutathione (GSH)-dependent disulfide- bond reductase activity toward the model substrate, 2-hydroxyethyl disulfide; the actual physiological substrates are not known. Also displays a modest GSH-dependent peroxidase activity toward several organic hydroperoxides, such as cumene hydroperoxide and linoleic acid 13(S)-hydroperoxide, but does not reduce H(2)O(2) or tert- butyl hydroperoxide at appreciable rates. Exhibits little or no GSH transferase activity with most typical electrophilic substrates, and has no detectable transferase activity toward 1- chloro-2,4-dinitrobenzene (CDNB) with glutathionylspermidine (GspSH) as the nucleophilic substrate. 

Sequence Annotation

 DOMAIN 46 133 GST N-terminal.
 DOMAIN 139 265 GST C-terminal.
 REGION 52 54 Glutathione 1 binding.
 REGION 117 118 Glutathione 1 binding.
 BINDING 26 26 Glutathione 2.
 BINDING 87 87 Glutathione 1.
 BINDING 101 101 Glutathione 1; via amide nitrogen and
 BINDING 151 151 Glutathione 1.
 BINDING 178 178 Glutathione 2.  

Keyword

 3D-structure; Complete proteome; Oxidoreductase; Peroxidase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 288 AA 

Protein Sequence

Gene Ontology

 GO:0015036; F:disulfide oxidoreductase activity; IDA:EcoCyc.
 GO:0004601; F:peroxidase activity; IEA:UniProtKB-KW. 

Interpro

 IPR010987; Glutathione-S-Trfase_C-like.
 IPR004045; Glutathione_S-Trfase_N.
 IPR017933; Glutathione_S_Trfase/Cl_chnl_C.
 IPR012336; Thioredoxin-like_fold. 

Pfam

  

SMART

  

PROSITE

 PS50405; GST_CTER
 PS50404; GST_NTER 

PRINTS