CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002724
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Malate synthase A 
Protein Synonyms/Alias
 MSA 
Gene Name
 aceB 
Gene Synonyms/Alias
 mas; b4014; JW3974 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
46HFTPQRNKLLAARIQacetylation[1, 2]
79SIRDADWKIRGIPADacetylation[2]
101ITGPVERKMVINALNacetylation[1, 2]
152SYTNEAGKIYQLKPNacetylation[2]
157AGKIYQLKPNPAVLIacetylation[1, 2]
175RGLHLPEKHVTWRGEacetylation[1, 2]
305RQAVTMDKPFLNAYSacetylation[2]
317AYSRLLIKTCHKRGAacetylation[1]
337MAAFIPSKDEEHNNQacetylation[1, 2]
348HNNQVLNKVKADKSLacetylation[2]
466WQWIHHQKTLSNGKPacetylation[1, 2]
472QKTLSNGKPVTKALFacetylation[1, 2]
476SNGKPVTKALFRQMLacetylation[2]
488QMLGEEMKVIASELGacetylation[2]
Reference
 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
  
Sequence Annotation
 ACT_SITE 166 166 Proton acceptor (By similarity).
 ACT_SITE 447 447 Proton donor (By similarity).  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Glyoxylate bypass; Reference proteome; Transferase; Tricarboxylic acid cycle. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 533 AA 
Protein Sequence
MTEQATTTDE LAFTRPYGEQ EKQILTAEAV EFLTELVTHF TPQRNKLLAA RIQQQQDIDN 60
GTLPDFISET ASIRDADWKI RGIPADLEDR RVEITGPVER KMVINALNAN VKVFMADFED 120
SLAPDWNKVI DGQINLRDAV NGTISYTNEA GKIYQLKPNP AVLICRVRGL HLPEKHVTWR 180
GEAIPGSLFD FALYFFHNYQ ALLAKGSGPY FYLPKTQSWQ EAAWWSEVFS YAEDRFNLPR 240
GTIKATLLIE TLPAVFQMDE ILHALRDHIV GLNCGRWDYI FSYIKTLKNY PDRVLPDRQA 300
VTMDKPFLNA YSRLLIKTCH KRGAFAMGGM AAFIPSKDEE HNNQVLNKVK ADKSLEANNG 360
HDGTWIAHPG LADTAMAVFN DILGSRKNQL EVMREQDAPI TADQLLAPCD GERTEEGMRA 420
NIRVAVQYIE AWISGNGCVP IYGLMEDAAT AEISRTSIWQ WIHHQKTLSN GKPVTKALFR 480
QMLGEEMKVI ASELGEERFS QGRFDDAARL MEQITTSDEL IDFLTLPGYR LLA 533 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:EcoliWiki.
 GO:0004474; F:malate synthase activity; IDA:EcoliWiki.
 GO:0006097; P:glyoxylate cycle; IC:EcoliWiki.
 GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW. 
Interpro
 IPR011076; Malate_synth-like.
 IPR006252; Malate_synthA.
 IPR001465; Malate_synthase.
 IPR019830; Malate_synthase_CS. 
Pfam
 PF01274; Malate_synthase 
SMART
  
PROSITE
 PS00510; MALATE_SYNTHASE 
PRINTS