CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006115
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcriptional activator of sulfur metabolism MET4 
Protein Synonyms/Alias
 Methionine-requiring protein 4 
Gene Name
 MET4 
Gene Synonyms/Alias
 YNL103W; N2177 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
163FIFPDEDKPSNNNNNubiquitination[1]
409ENNSNNNKNINTNDSubiquitination[2]
Reference
 [1] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Positive trans-acting factor capable of stimulating the transcription of the MET genes from the methionine biosynthetic pathway. MET4, MET28 and CBF1 are required for full induction of MET25 and MET16 gene transcription. MET4 controls as well the derepression of MET6. Required for the transcription of genes necessary for sulfur amino acid biosynthesis. Involved in the transcription activation of MET28 and MET30. Required for MET3 gene expression via assembly of the MET4-MET28-MET31 and MET4- MET28-MET32 complexes. Involved in response to cadmium and arsenic. Cadmium-activated MET4 also induces glutathione biosynthesis. 
Sequence Annotation
 DOMAIN 586 649 bZIP.
 REGION 95 144 Transcriptional activation.
 REGION 188 235 Inhibitory region; AdoMet responsiveness;
 REGION 312 375 Auxiliary; required for high
 REGION 375 403 Required for interaction with MET31 and
 REGION 601 612 Basic motif (By similarity).
 REGION 614 642 Leucine-zipper (By similarity).
 MOD_RES 416 416 Phosphoserine.
 MOD_RES 564 564 Phosphoserine.  
Keyword
 Activator; Amino-acid biosynthesis; Coiled coil; Complete proteome; Cysteine biosynthesis; DNA-binding; Methionine biosynthesis; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 672 AA 
Protein Sequence
MKQEQSHEGD SYSTEFINLF GKDTATHPSS NNGANNNGMG STNSLDQFVA TASSSSSLVT 60
SSENRRPLIG DVTNRGNTNL YDHAVTPEIL LEQLAYVDNF IPSLDNEFSN VDWNVNTTHN 120
NANNNGADTF SSINANPFDL DEQLAIELSA FADDSFIFPD EDKPSNNNNN SNNGNDDHSN 180
HDVLHEDPST NNRQRNPHFL TQRRNTFLTS QYDQSKSRFS SKNKRNGNNG ETNNFGDNMQ 240
NNHPFEPNFM GSPSQFPADA TNMTSIDHGG FTNVDITSTE NNTTGDNGVD ALSNLLHRTT 300
HTPNRSSPLS NVTSAQNSSS QQRKHSESKV DSNSDNNSSN KAPNITVPDY SIIPTSVLVT 360
LLPRVNVPNG AYNSLISAGF DNDQIDAIAA IMAYHHQKKI RENNSNNNKN INTNDSQEAP 420
ILKNINELLS VLIPPSPAET RGPTTLSTSP SFNEHGVVAE ASFLSSILEL GIKHPKSNNI 480
HNQRQPSRND HKISRESDGN NGNDNVHHNN AVIKSSTTRG DEIAKIRSEP TLNASSSDHK 540
ENSLKRSHSG DLKNKKVPVD RKYSDNEDDE YDDADLHGFE KKQLIKKELG DDDEDLLIQS 600
KKSHQKKKLK EKELESSIHE LTEIAASLQK RIHTLETENK LLKNLVLSSG ETEGIKKAES 660
LKKQIFEKVQ KE 672 
Gene Ontology
 GO:0005634; C:nucleus; IPI:SGD.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
 GO:0003713; F:transcription coactivator activity; IDA:SGD.
 GO:0019344; P:cysteine biosynthetic process; IEA:UniProtKB-KW.
 GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:SGD.
 GO:0046685; P:response to arsenic-containing substance; IDA:SGD.
 GO:0046686; P:response to cadmium ion; IDA:SGD.
 GO:0000096; P:sulfur amino acid metabolic process; IMP:SGD.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR004827; bZIP. 
Pfam
  
SMART
 SM00338; BRLZ 
PROSITE
 PS50217; BZIP
 PS00036; BZIP_BASIC 
PRINTS