UniProt Accession

 NEP1_MOUSE; O35130 

Genbank Protein ID

 AC002397; BC002004 

Genbank Nucleotide ID

 AAC36006.1; AAH02004.1 

Protein Name

 Ribosomal RNA small subunit methyltransferase NEP1 

Protein Synonyms/Alias

 18S rRNA (pseudouridine-N1-)-methyltransferase NEP1; Nucleolar protein EMG1 homolog; Protein C2f; Ribosome biogenesis protein NEP1 

Gene Name

 Emg1 

Gene Synonyms/Alias

 C2f; Grcc2f 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
59	LETVKVGKTYELLNC	ubiquitination	[1]
154	RAADGPQKLLKVIKN	ubiquitination	[1]
160	QKLLKVIKNPVSDHF	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Involved in 40S ribosomal subunit biogenesis and 18S rRNA processing. Specifically catalyzes the N1-methylation of the pseudouridine corresponding to position 1248 in human 18S rRNA. Thus, appears to be the methyltransferase involved in the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3- carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA (By similarity). 

Sequence Annotation

 REGION 219 224 S-adenosyl-L-methionine binding (By
 BINDING 201 201 S-adenosyl-L-methionine; via amide
 BINDING 206 206 S-adenosyl-L-methionine; via carbonyl
 MOD_RES 16 16 Phosphoserine.  

Keyword

 Complete proteome; Methyltransferase; Nucleus; Phosphoprotein; Reference proteome; Ribosome biogenesis; RNA-binding; rRNA processing; rRNA-binding; S-adenosyl-L-methionine; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 244 AA 

Protein Sequence

Gene Ontology

 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0070037; F:rRNA (pseudouridine) methyltransferase activity; ISS:UniProtKB.
 GO:0019843; F:rRNA binding; IEA:UniProtKB-KW. 

Interpro

 IPR005304; Rbsml_bgen_MeTrfase_EMG1/NEP1. 

Pfam

 PF03587; EMG1 

SMART

  

PROSITE

  

PRINTS