CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030551
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E3 SUMO-protein ligase PIAS1 
Protein Synonyms/Alias
 cDNA FLJ37322 fis, clone BRAMY2018126, highly similar to Protein inhibitor of activated STAT protein 1 
Gene Name
 PIAS1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
48TKALHLLKAGCSPAVubiquitination[1, 2, 3, 4]
58CSPAVQMKIKELYRRubiquitination[3]
60PAVQMKIKELYRRRFubiquitination[3]
142HPDIKLQKLPFYDLLubiquitination[3]
228LCVKVNTKPCSLPGYubiquitination[3, 4]
240PGYLPPTKNGVEPKRubiquitination[3, 4]
315DHSRALIKEKLTADPubiquitination[3]
317SRALIKEKLTADPDSubiquitination[2, 3, 4, 5]
341SLLCPLGKMRLTIPCubiquitination[1, 3]
410DCDEIQFKEDGTWAPubiquitination[1, 3]
421TWAPMRSKKEVQEVSubiquitination[3]
422WAPMRSKKEVQEVSAubiquitination[1]
455SHHQSSNKNKKVEVIubiquitination[1, 3]
457HQSSNKNKKVEVIDLubiquitination[3]
458QSSNKNKKVEVIDLTubiquitination[3]
495PTSPLNNKGILSLPHubiquitination[1, 3, 4]
Reference
 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 653 AA 
Protein Sequence
MFTLQDSYVK QMVMSLRVSE LQVLLGYAGR NKHGRKHELL TKALHLLKAG CSPAVQMKIK 60
ELYRRRFPQK IMTPADLSIP NVHSSPMPAT LSPSTIPQLT YDGHPASSPL LPVSLLGPKH 120
ELELPHLTSA LHPVHPDIKL QKLPFYDLLD ELIKPTSLAS DNSQRFRETC FAFALTPQQV 180
QQISSSMDIS GTKCDFTVQV QLRFCLSETS CPQEDHFPPN LCVKVNTKPC SLPGYLPPTK 240
NGVEPKRPSR PINITSLVRL STTVPNTIVV SWTAEIGRNY SMAVYLVKQL SSTVLLQRLR 300
AKGIRNPDHS RALIKEKLTA DPDSEIATTS LRVSLLCPLG KMRLTIPCRA LTCSHLQCFD 360
ATLYIQMNEK KPTWVCPVCD KKAPYEHLII DGLFMEILKY CTDCDEIQFK EDGTWAPMRS 420
KKEVQEVSAS YNGVDGCLSS TLEHQVASHH QSSNKNKKVE VIDLTIDSSS DEEEEEPSAK 480
RTCPSLSPTS PLNNKGILSL PHQASPVSRT PSLPAVDTSY INTSLIQDYR HPFHMTPMPY 540
DLQGLDFFPF LSGDNQHYNT SLLAAAAAAV SDDQDLLHSS RFFPYTSSQM FLDQLSAGGS 600
TSLPTTNGSS SGSNSSLVSS NSLRESHSHT VTNRSSTDTA SIFGIIPDII SLD 653 
Gene Ontology
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0019789; F:SUMO ligase activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR027227; PIAS1.
 IPR023321; PINIT.
 IPR003034; SAP_dom.
 IPR004181; Znf_MIZ. 
Pfam
 PF14324; PINIT
 PF02891; zf-MIZ 
SMART
 SM00513; SAP 
PROSITE
 PS51466; PINIT
 PS50800; SAP
 PS51044; ZF_SP_RING 
PRINTS