CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007672
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Melanoma-associated antigen 12 
Protein Synonyms/Alias
 Cancer/testis antigen 1.12; CT1.12; MAGE-12 antigen; MAGE12F antigen 
Gene Name
 MAGEA12 
Gene Synonyms/Alias
 MAGE12 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
112FQVALSRKMAELVHFubiquitination[1]
132RAREPFTKAEMLGSVubiquitination[1, 2, 3]
220GDCAPEEKIWEELSVubiquitination[1]
244SVFAHPRKLLTQDLVubiquitination[1, 2, 3, 4]
285LVETSYVKVLHHLLKubiquitination[1]
Reference
 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048
Functional Description
 Not known, though may play a role tumor transformation or progression. In vitro promotes cell viability in melanoma cell lines. 
Sequence Annotation
 DOMAIN 109 308 MAGE.  
Keyword
 Complete proteome; Polymorphism; Reference proteome; Tumor antigen. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 314 AA 
Protein Sequence
MPLEQRSQHC KPEEGLEAQG EALGLVGAQA PATEEQETAS SSSTLVEVTL REVPAAESPS 60
PPHSPQGAST LPTTINYTLW SQSDEGSSNE EQEGPSTFPD LETSFQVALS RKMAELVHFL 120
LLKYRAREPF TKAEMLGSVI RNFQDFFPVI FSKASEYLQL VFGIEVVEVV RIGHLYILVT 180
CLGLSYDGLL GDNQIVPKTG LLIIVLAIIA KEGDCAPEEK IWEELSVLEA SDGREDSVFA 240
HPRKLLTQDL VQENYLEYRQ VPGSDPACYE FLWGPRALVE TSYVKVLHHL LKISGGPHIS 300
YPPLHEWAFR EGEE 314 
Gene Ontology
  
Interpro
 IPR002190; MAGE.
 IPR021072; Melanoma_ass_antigen_N. 
Pfam
 PF01454; MAGE
 PF12440; MAGE_N 
SMART
  
PROSITE
 PS50838; MAGE 
PRINTS