| Tag | Content |
|---|
| CPLM ID | CPLM-010041 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Phosphoenolpyruvate-protein phosphotransferase |
Protein Synonyms/Alias | Phosphotransferase system, enzyme I |
Gene Name | ptsI |
Gene Synonyms/Alias | MPN_627; MP215 |
Created Date | July 27, 2013 |
Organism | Mycoplasma pneumoniae (strain ATCC 29342 / M129) |
NCBI Taxa ID | 272634 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 30 | TPEFAVNKYLKHQLT | acetylation | [1] | | 146 | LAYLTGQKPHDLVKI | acetylation | [1] | | 207 | IPAVVGIKGITTKVK | acetylation | [1] | | 241 | SKDTTEWKKQKALEE | acetylation | [1] | | 249 | KQKALEEKYQQELKQ | acetylation | [1] | | 559 | ANAKEVEKLVEKFFK | acetylation | [1] | | 563 | EVEKLVEKFFKKKDI | acetylation | [1] |
|
Reference | [1] Cross-talk between phosphorylation and lysine acetylation in a genome-reduced bacterium. van Noort V, Seebacher J, Bader S, Mohammed S, Vonkova I, Betts MJ, Kühner S, Kumar R, Maier T, O'Flaherty M, Rybin V, Schmeisky A, Yus E, Stülke J, Serrano L, Russell RB, Heck AJ, Bork P, Gavin AC. Mol Syst Biol. 2012 Feb 28;8:571. [ PMID: 22373819] |
Functional Description | General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr) (By similarity). |
Sequence Annotation | ACT_SITE 190 190 Tele-phosphohistidine intermediate (By
ACT_SITE 498 498 Proton donor (By similarity).
METAL 427 427 Magnesium (By similarity).
METAL 451 451 Magnesium (By similarity).
BINDING 297 297 Substrate (By similarity).
BINDING 333 333 Substrate (By similarity).
BINDING 427 427 Substrate (By similarity).
BINDING 448 448 Substrate; via carbonyl oxygen (By
BINDING 449 449 Substrate; via amide nitrogen (By
BINDING 450 450 Substrate (By similarity).
BINDING 451 451 Substrate; via amide nitrogen (By |
Keyword | Complete proteome; Cytoplasm; Kinase; Magnesium; Metal-binding; Phosphotransferase system; Reference proteome; Sugar transport; Transferase; Transport. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 572 AA |
Protein Sequence | MKKLSGIGVS DGMALAKAFL VKTPEFAVNK YLKHQLTKAQ AKRLLDSAFK KAVKDLEEIK 60
EITVNNINTE AGMIFDAHIQ MLNDPTITEQ LEQQLAQNVH PVIAVDTVFS QTATMFSQMQ 120
DKYFQERAAD ILDLRQRLLA YLTGQKPHDL VKIKSDVIIV AHDLTPSQTA TLNKKYVKGF 180
LTEIGGRTSH AAIMARSLEI PAVVGIKGIT TKVKDGQIVG VDGRKGIAGL DLNSKDTTEW 240
KKQKALEEKY QQELKQYTNK ETVTLDGHAV VVAANIGNVK DMELACQYNT NGVGLFRTEF 300
LYMNSQEWPD EETQYQAYKA VLEQAHGDLV IIRTLDIGGD KKLNYYEFPH EDNPFLGYRA 360
LRLTLDKQDI FKTQLRALLR AADHGQLGIM FPMVATLDEL LQAKQLLNQV HQELGGNKQF 420
KLGIMIEIPA AVLAANTLSH HVDFFSIGTN DLIQYSFAAD RMNKNVSYLY QPLNPALLKL 480
IYLTIEGGKV NDIWTGMCGE MAGEPLAIPL LLGLGLKEFS MSASSMFKAR MIIAKLNYTE 540
CQTLAQKALT LANAKEVEKL VEKFFKKKDI FI 572 |
Gene Ontology | GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. GO:0016301; F:kinase activity; IEA:UniProtKB-KW. GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. GO:0008965; F:phosphoenolpyruvate-protein phosphotransferase activity; IEA:EC. GO:0005351; F:sugar:hydrogen symporter activity; IEA:InterPro. GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |