CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019481
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Dedicator of cytokinesis protein 7 
Protein Synonyms/Alias
  
Gene Name
 DOCK7 
Gene Synonyms/Alias
 KIAA1771 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
177QDDQDDLKRRSMSIDubiquitination[1, 2, 3]
233QRKSNRHKELFALHPubiquitination[3]
366AEPYMIFKEADATKNubiquitination[3]
532TPELLQVKLYPDSRVubiquitination[2, 3]
1962GELHEQFKRKTILTTacetylation[4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 Functions as a guanine nucleotide exchange factor (GEF), which activates Rac1 and Rac3 Rho small GTPases by exchanging bound GDP for free GTP. Does not have a GEF activity for CDC42. Required for STMN1 'Ser-15' phosphorylation during axon formation and consequently for neuronal polarization. 
Sequence Annotation
 DOMAIN 561 727 DHR-1.
 DOMAIN 1678 2114 DHR-2.
 MOD_RES 28 28 Phosphoserine.
 MOD_RES 30 30 Phosphoserine.
 MOD_RES 180 180 Phosphoserine.
 MOD_RES 182 182 Phosphoserine.
 MOD_RES 439 439 Phosphoserine (By similarity).
 MOD_RES 440 440 Phosphoserine (By similarity).
 MOD_RES 882 882 Phosphoserine.
 MOD_RES 888 888 Phosphoserine.
 MOD_RES 896 896 Phosphoserine.
 MOD_RES 900 900 Phosphoserine.
 MOD_RES 905 905 Phosphoserine.
 MOD_RES 907 907 Phosphothreonine.
 MOD_RES 909 909 Phosphothreonine.
 MOD_RES 910 910 Phosphoserine.
 MOD_RES 964 964 Phosphoserine (By similarity).
 MOD_RES 966 966 Phosphothreonine (By similarity).
 MOD_RES 1383 1383 Phosphoserine.
 MOD_RES 1430 1430 Phosphoserine.
 MOD_RES 1432 1432 Phosphoserine.
 MOD_RES 1438 1438 Phosphoserine.
 MOD_RES 1962 1962 N6-acetyllysine.  
Keyword
 Acetylation; Alternative splicing; Cell projection; Coiled coil; Complete proteome; Developmental protein; Differentiation; Guanine-nucleotide releasing factor; Neurogenesis; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2140 AA 
Protein Sequence
MAERRAFAQK ISRTVAAEVR KQISGQYSGS PQLLKNLNIV GNISHHTTVP LTEAVDPVDL 60
EDYLITHPLA VDSGPLRDLI EFPPDDIEVV YSPRDCRTLV SAVPEESEMD PHVRDCIRSY 120
TEDWAIVIRK YHKLGTGFNP NTLDKQKERQ KGLPKQVFES DEAPDGNSYQ DDQDDLKRRS 180
MSIDDTPRGS WACSIFDLKN SLPDALLPNL LDRTPNEEID RQNDDQRKSN RHKELFALHP 240
SPDEEEPIER LSVPDIPKEH FGQRLLVKCL SLKFEIEIEP IFASLALYDV KEKKKISENF 300
YFDLNSEQMK GLLRPHVPPA AITTLARSAI FSITYPSQDV FLVIKLEKVL QQGDIGECAE 360
PYMIFKEADA TKNKEKLEKL KSQADQFCQR LGKYRMPFAW TAIHLMNIVS SAGSLERDST 420
EVEISTGERK GSWSERRNSS IVGRRSLERT TSGDDACNLT SFRPATLTVT NFFKQEGDRL 480
SDEDLYKFLA DMRRPSSVLR RLRPITAQLK IDISPAPENP HYCLTPELLQ VKLYPDSRVR 540
PTREILEFPA RDVYVPNTTY RNLLYIYPQS LNFANRQGSA RNITVKVQFM YGEDPSNAMP 600
VIFGKSSCSE FSKEAYTAVV YHNRSPDFHE EIKVKLPATL TDHHHLLFTF YHVSCQQKQN 660
TPLETPVGYT WIPMLQNGRL KTGQFCLPVS LEKPPQAYSV LSPEVPLPGM KWVDNHKGVF 720
NVEVVAVSSI HTQDPYLDKF FALVNALDEH LFPVRIGDMR IMENNLENEL KSSISALNSS 780
QLEPVVRFLH LLLDKLILLV IRPPVIAGQI VNLGQASFEA MASIINRLHK NLEGNHDQHG 840
RNSLLASYIH YVFRLPNTYP NSSSPGPGGL GGSVHYATMA RSAVRPASLN LNRSRSLSNS 900
NPDISGTPTS PDDEVRSIIG SKGLDRSNSW VNTGGPKAAP WGSNPSPSAE STQAMDRSCN 960
RMSSHTETSS FLQTLTGRLP TKKLFHEELA LQWVVCSGSV RESALQQAWF FFELMVKSMV 1020
HHLYFNDKLE APRKSRFPER FMDDIAALVS TIASDIVSRF QKDTEMVERL NTSLAFFLND 1080
LLSVMDRGFV FSLIKSCYKQ VSSKLYSLPN PSVLVSLRLD FLRIICSHEH YVTLNLPCSL 1140
LTPPASPSPS VSSATSQSSG FSTNVQDQKI ANMFELSVPF RQQHYLAGLV LTELAVILDP 1200
DAEGLFGLHK KVINMVHNLL SSHDSDPRYS DPQIKARVAM LYLPLIGIIM ETVPQLYDFT 1260
ETHNQRGRPI CIATDDYESE SGSMISQTVA MAIAGTSVPQ LTRPGSFLLT STSGRQHTTF 1320
SAESSRSLLI CLLWVLKNAD ETVLQKWFTD LSVLQLNRLL DLLYLCVSCF EYKGKKVFER 1380
MNSLTFKKSK DMRAKLEEAI LGSIGARQEM VRRSRGQLGT YTIASPPERS PSGSAFGSQE 1440
NLRWRKDMTH WRQNTEKLDK SRAEIEHEAL IDGNLATEAN LIILDTLEIV VQTVSVTESK 1500
ESILGGVLKV LLHSMACNQS AVYLQHCFAT QRALVSKFPE LLFEEETEQC ADLCLRLLRH 1560
CSSSIGTIRS HASASLYLLM RQNFEIGNNF ARVKMQVTMS LSSLVGTSQN FNEEFLRRSL 1620
KTILTYAEED LELRETTFPD QVQDLVFNLH MILSDTVKMK EHQEDPEMLI DLMYRIAKGY 1680
QTSPDLRLTW LQNMAGKHSE RSNHAEAAQC LVHSAALVAE YLSMLEDRKY LPVGCVTFQN 1740
ISSNVLEESA VSDDVVSPDE EGICSGKYFT ESGLVGLLEQ AAASFSMAGM YEAVNEVYKV 1800
LIPIHEANRD AKKLSTIHGK LQEAFSKIVH QSTGWERMFG TYFRVGFYGT KFGDLDEQEF 1860
VYKEPAITKL AEISHRLEGF YGERFGEDVV EVIKDSNPVD KCKLDPNKAY IQITYVEPYF 1920
DTYEMKDRIT YFDKNYNLRR FMYCTPFTLD GRAHGELHEQ FKRKTILTTS HAFPYIKTRV 1980
NVTHKEEIIL TPIEVAIEDM QKKTQELAFA THQDPADPKM LQMVLQGSVG TTVNQGPLEV 2040
AQVFLSEIPS DPKLFRHHNK LRLCFKDFTK RCEDALRKNK SLIGPDQKEY QRELERNYHR 2100
LKEALQPLIN RKIPQLYKAV LPVTCHRDSF SRMSLRKMDL 2140 
Gene Ontology
 GO:0030424; C:axon; TAS:BHF-UCL.
 GO:0045178; C:basal part of cell; TAS:BHF-UCL.
 GO:0030426; C:growth cone; TAS:BHF-UCL.
 GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
 GO:0048365; F:Rac GTPase binding; IDA:BHF-UCL.
 GO:0032863; P:activation of Rac GTPase activity; IDA:BHF-UCL.
 GO:0007409; P:axonogenesis; IMP:BHF-UCL.
 GO:0045200; P:establishment of neuroblast polarity; IMP:BHF-UCL.
 GO:0000226; P:microtubule cytoskeleton organization; IMP:BHF-UCL.
 GO:0043473; P:pigmentation; IEA:Compara.
 GO:0033138; P:positive regulation of peptidyl-serine phosphorylation; IMP:BHF-UCL.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. 
Interpro
 IPR027007; DHR-1_domain.
 IPR027357; DHR-2.
 IPR026791; DOCK.
 IPR010703; DOCK_C.
 IPR021816; DOCK_C/D_N.
 IPR026798; DOCK_C_fam. 
Pfam
 PF06920; Ded_cyto
 PF14429; DOCK-C2
 PF11878; DUF3398 
SMART
  
PROSITE
 PS51650; DHR_1
 PS51651; DHR_2 
PRINTS