UniProt Accession

 GSH0_HUMAN; P48507; A8K334; D3DT45; Q6FHC1; Q9NPX9; Q9NU74 

Genbank Protein ID

 L35546; CR541833; AY773965; AK290449; AL049796; AL117351; AL117351; AL049796; CH471097; CH471097; BC041809 

Genbank Nucleotide ID

 AAA65028.1; CAG46632.1; AAV28733.1; BAF83138.1; CAI21813.1; CAI21813.1; CAI22976.1; CAI22976.1; EAW73060.1; EAW73061.1; AAH41809.1 

Protein Name

 Glutamate--cysteine ligase regulatory subunit 

Protein Synonyms/Alias

 GCS light chain; Gamma-ECS regulatory subunit; Gamma-glutamylcysteine synthetase regulatory subunit; Glutamate--cysteine ligase modifier subunit 

Gene Name

 GCLM 

Gene Synonyms/Alias

 GLCLR 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
9	GTDSRAAKALLARAR	ubiquitination	[1]
33	LNWGRLRKKCPSTHS	ubiquitination	[1]
90	PDEREEMKVSAKLFI	ubiquitination	[1]
263	SRGIIKSKGYILQAK	acetylation	[2, 3]
263	SRGIIKSKGYILQAK	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773] 

Functional Description

  

Sequence Annotation

 MOD_RES 263 263 N6-acetyllysine.  

Keyword

 Acetylation; Complete proteome; Glutathione biosynthesis; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 274 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; NAS:UniProtKB.
 GO:0017109; C:glutamate-cysteine ligase complex; IEA:Compara.
 GO:0006534; P:cysteine metabolic process; IEA:Compara.
 GO:0006536; P:glutamate metabolic process; IDA:UniProtKB.
 GO:0006750; P:glutathione biosynthetic process; IDA:UniProtKB.
 GO:0043524; P:negative regulation of neuron apoptotic process; IEA:Compara.
 GO:0035229; P:positive regulation of glutamate-cysteine ligase activity; IEA:Compara.
 GO:0050880; P:regulation of blood vessel size; IMP:UniProtKB.
 GO:0051900; P:regulation of mitochondrial depolarization; IEA:Compara.
 GO:0042493; P:response to drug; IDA:UniProtKB.
 GO:0051409; P:response to nitrosative stress; IEA:Compara.
 GO:0006979; P:response to oxidative stress; IDA:UniProtKB.
 GO:0000096; P:sulfur amino acid metabolic process; TAS:Reactome.
 GO:0006805; P:xenobiotic metabolic process; TAS:Reactome. 

Interpro

 IPR023210; NADP_OxRdtase_dom. 

Pfam

 PF00248; Aldo_ket_red 

SMART

  

PROSITE

  

PRINTS