CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021311
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Activity-dependent neuroprotector homeobox protein 
Protein Synonyms/Alias
 Activity-dependent neuroprotective protein 
Gene Name
 ADNP 
Gene Synonyms/Alias
 ADNP1; KIAA0784 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
126KTLETHIKIFHAPNAubiquitination[1, 2]
199VAAPYIAKAGEKSLNacetylation[3]
621PYKKDVGKTLCPLCFubiquitination[4, 5]
716SPSLAPVKRTYEQMEubiquitination[5]
773ARKSFLTKYFNKQPYubiquitination[1, 2]
777FLTKYFNKQPYPTRRubiquitination[1, 2]
807IASHFSNKRKKCVRDubiquitination[4]
829VLLGFNMKELNKVKHubiquitination[1, 2]
1035WKNSSYGKVEGFWSKacetylation[6]
1042KVEGFWSKDQSQWKNacetylation[6]
1048SKDQSQWKNASENDEubiquitination[7]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [7] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983
Functional Description
 Potential transcription factor. May mediate some of the neuroprotective peptide VIP-associated effects involving normal growth and cancer proliferation. 
Sequence Annotation
 ZN_FING 74 97 C2H2-type 1.
 ZN_FING 107 129 C2H2-type 2; atypical.
 ZN_FING 165 188 C2H2-type 3.
 ZN_FING 221 244 C2H2-type 4.
 ZN_FING 447 469 C2H2-type 5; atypical.
 ZN_FING 489 510 C2H2-type 6; atypical.
 ZN_FING 512 535 C2H2-type 7.
 ZN_FING 622 647 C2H2-type 8; atypical.
 ZN_FING 662 686 C2H2-type 9; atypical.
 DNA_BIND 754 814 Homeobox.
 REGION 354 361 Neuroprotective peptide (NAP).
 MOD_RES 98 98 Phosphoserine.
 MOD_RES 409 409 Phosphoserine.
 MOD_RES 413 413 Phosphoserine.
 MOD_RES 709 709 Phosphoserine.
 MOD_RES 736 736 Phosphoserine.
 MOD_RES 805 805 Phosphoserine.
 MOD_RES 876 876 Phosphoserine.
 MOD_RES 878 878 Phosphoserine.
 MOD_RES 921 921 Phosphoserine.
 MOD_RES 953 953 Phosphoserine.
 MOD_RES 955 955 Phosphoserine.
 MOD_RES 1035 1035 N6-acetyllysine.
 MOD_RES 1042 1042 N6-acetyllysine.  
Keyword
 Acetylation; Complete proteome; DNA-binding; Homeobox; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1102 AA 
Protein Sequence
MFQLPVNNLG SLRKARKTVK KILSDIGLEY CKEHIEDFKQ FEPNDFYLKN TTWEDVGLWD 60
PSLTKNQDYR TKPFCCSACP FSSKFFSAYK SHFRNVHSED FENRILLNCP YCTFNADKKT 120
LETHIKIFHA PNASAPSSSL STFKDKNKND GLKPKQADSV EQAVYYCKKC TYRDPLYEIV 180
RKHIYREHFQ HVAAPYIAKA GEKSLNGAVP LGSNAREESS IHCKRCLFMP KSYEALVQHV 240
IEDHERIGYQ VTAMIGHTNV VVPRSKPLML IAPKPQDKKS MGLPPRIGSL ASGNVRSLPS 300
QQMVNRLSIP KPNLNSTGVN MMSSVHLQQN NYGVKSVGQG YSVGQSMRLG LGGNAPVSIP 360
QQSQSVKQLL PSGNGRSYGL GSEQRSQAPA RYSLQSANAS SLSSGQLKSP SLSQSQASRV 420
LGQSSSKPAA AATGPPPGNT SSTQKWKICT ICNELFPENV YSVHFEKEHK AEKVPAVANY 480
IMKIHNFTSK CLYCNRYLPT DTLLNHMLIH GLSCPYCRST FNDVEKMAAH MRMVHIDEEM 540
GPKTDSTLSF DLTLQQGSHT NIHLLVTTYN LRDAPAESVA YHAQNNPPVP PKPQPKVQEK 600
ADIPVKSSPQ AAVPYKKDVG KTLCPLCFSI LKGPISDALA HHLRERHQVI QTVHPVEKKL 660
TYKCIHCLGV YTSNMTASTI TLHLVHCRGV GKTQNGQDKT NAPSRLNQSP SLAPVKRTYE 720
QMEFPLLKKR KLDDDSDSPS FFEEKPEEPV VLALDPKGHE DDSYEARKSF LTKYFNKQPY 780
PTRREIEKLA ASLWLWKSDI ASHFSNKRKK CVRDCEKYKP GVLLGFNMKE LNKVKHEMDF 840
DAEWLFENHD EKDSRVNASK TADKKLNLGK EDDSSSDSFE NLEEESNESG SPFDPVFEVE 900
PKISNDNPEE HVLKVIPEDA SESEEKLDQK EDGSKYETIH LTEEPTKLMH NASDSEVDQD 960
DVVEWKDGAS PSESGPGSQQ VSDFEDNTCE MKPGTWSDES SQSEDARSSK PAAKKKATMQ 1020
GDREQLKWKN SSYGKVEGFW SKDQSQWKNA SENDERLSNP QIEWQNSTID SEDGEQFDNM 1080
TDGVAEPMHG SLAGVKLSSQ QA 1102 
Gene Ontology
 GO:0005615; C:extracellular space; IEA:Compara.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0043524; P:negative regulation of neuron apoptotic process; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR001356; Homeodomain.
 IPR009057; Homeodomain-like.
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like. 
Pfam
 PF00046; Homeobox 
SMART
 SM00389; HOX
 SM00355; ZnF_C2H2 
PROSITE
 PS00027; HOMEOBOX_1
 PS50071; HOMEOBOX_2
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS