CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-039458
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Ras GTPase-activating-like protein IQGAP3 
Protein Synonyms/Alias
  
Gene Name
 IQGAP3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
271QSPEALLKALQDPALubiquitination[1]
776RRLHYFQKNVNSIVKubiquitination[2]
968YLLLQLFKTALQEEIubiquitination[1, 3]
1024IQDVLEDKVLSVHTDubiquitination[2]
1115LKATLAEKFPDATDSubiquitination[2]
1202YLEETHLKFRKFIHRubiquitination[2]
1359REQEAAHKQLMSRRQubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1588 AA 
Protein Sequence
MEACLKEELP SPVELEESLR NGVLLAKLGH CFAPSVVPLK KIYDVEQLRY QATGLHFRHT 60
DNINFWLSAI AHIGLPSTFF PETTDIYDKK NMPRVVYCIH ALSLFLFRLG LAPQIHDLYG 120
KVKFTAEELS NMASELAKYG LQLPAFSKIG GILANELSVD EAAVHAAVLA INEAVERGVV 180
EDTLAALQNP SALLENLREP LAAVYQEMLA QAKMEKAANA RNHDDRESQD IYDHYLTQAE 240
IQGNINHVNV HGALEVVDDA LERQSPEALL KALQDPALAL RGVRRDFADW YLEQLNSDRE 300
QKAQELGLVE LLEKEEVQAG VAAANTKGDQ EQAMLHAVQR INKAIRRRVA ADTVKELMCP 360
EAQLPPVYPV ASSMYQLELA VLQQQQGELG QEELFVAVEM LSAVVLINRA LEARDASGFW 420
SSLVNPATGL AEVEGENAQR YFDALLKLRQ ERGMGEDFLS WNDLQATVSQ VNAQTQEETD 480
RVLAVSLINE ALDKGSPEKT LSALLLPAAG LDDVSLPVAP RYHLLLVAAK RQKAQVTGDP 540
GAVLWLEEIR QGVVRANQDT NTAQRMALGV AAINQAIKEG KAAQTERVLR NPAVALRGVV 600
PDCANGYQRA LESAMAKKQR PADTAFWVQH DMKDGTAYYF HLQTFQGIWE QPPGCPLNTS 660
HLTREEIQSA VTKVTAAYDR QQLWKANVGF VIQLQARLRG FLVRQKFAEH SHFLRTWLPA 720
VIKIQAHWRG YRQRKIYLEW LQYFKANLDA IIKIQAWARM WAARRQYLRR LHYFQKNVNS 780
IVKIQAFFRA RKAQDDYRIL VHAPHPPLSV VRRFAHLLNQ SQQDFLAEAE LLKLQEEVVR 840
KIRSNQQLEQ DLNIMDIKIG LLVKNRITLQ EVVSHCKKLT KRNKEQLSDM MVLDKQKGLK 900
SLSKEKRQKL EAYQHLFYLL QTQPIYLAKL IFQMPQNKTT KFMEAVIFSL YNYASSRREA 960
YLLLQLFKTA LQEEIKSKVE QPQDVVTGNP TVVRLVVRFY RNGRGQSALQ EILGKVIQDV 1020
LEDKVLSVHT DPVHLYKNWI NQTEAQTGQR SHLPYDVTPE QALSHPEVQR RLDIALRNLL 1080
AMTDKFLLAI TSSVDQIPYG MRYVAKVLKA TLAEKFPDAT DSEVYKVVGN LLYYRFLNPA 1140
VVAPDAFDIV AMAAGGALAA PQRHALGAVA QLLQHAAAGK AFSGQSQHLR VLNDYLEETH 1200
LKFRKFIHRA CQVPEPEERF AVDEYSDMVA VAKPMVYITV GELVNTHRLL LEHQDCIAPD 1260
HQDPLHELLE DLGELPTIPD LIGESIAADG HTDLSKLEVS LTLTNKFEGL EADADDSNTR 1320
SLLLSTKQLL ADIIQFHPGD TLKEILSLSA SREQEAAHKQ LMSRRQACTA QTPEPLRRHR 1380
SLTAHSLLPL AEKQRRVLRN LRRLEALGLV SARNGYQGLV DELAKDIRNQ HRHRHRRKAE 1440
LVKLQATLQG LSTKTTFYEE QGDYYSQYIR ACLDHLAPDS KSSGKGKKQP SLHYTAAQLL 1500
EKGVLVEIED LPASHFRNVI FDITPGDEAG KFEVNAKFLG VDMERFQLHY QDLLQLQYEG 1560
VAVMKLFNKA KVNVNLLIFL LNKKFLRK 1588 
Gene Ontology
 GO:0005911; C:cell-cell junction; IEA:Compara.
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0016328; C:lateral plasma membrane; IEA:Compara.
 GO:0005099; F:Ras GTPase activator activity; IEA:InterPro.
 GO:0000187; P:activation of MAPK activity; IEA:Compara.
 GO:0071310; P:cellular response to organic substance; IEA:Compara.
 GO:0070371; P:ERK1 and ERK2 cascade; IEA:Compara.
 GO:0000082; P:G1/S transition of mitotic cell cycle; IEA:Compara.
 GO:0010629; P:negative regulation of gene expression; IEA:Compara.
 GO:0010628; P:positive regulation of gene expression; IEA:Compara.
 GO:0033601; P:positive regulation of mammary gland epithelial cell proliferation; IEA:Compara.
 GO:0032320; P:positive regulation of Ras GTPase activity; IEA:GOC.
 GO:0007265; P:Ras protein signal transduction; IEA:Compara.
 GO:0008361; P:regulation of cell size; IEA:Compara. 
Interpro
 IPR001715; CH-domain.
 IPR000048; IQ_motif_EF-hand-BS.
 IPR027417; P-loop_NTPase.
 IPR001936; RasGAP.
 IPR000593; RasGAP_C.
 IPR023152; RasGAP_CS.
 IPR008936; Rho_GTPase_activation_prot. 
Pfam
 PF00307; CH
 PF00612; IQ
 PF00616; RasGAP
 PF03836; RasGAP_C 
SMART
 SM00033; CH
 SM00015; IQ
 SM00323; RasGAP 
PROSITE
 PS50021; CH
 PS50096; IQ
 PS00509; RAS_GTPASE_ACTIV_1
 PS50018; RAS_GTPASE_ACTIV_2 
PRINTS