CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023735
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nitric oxide synthase-interacting protein 
Protein Synonyms/Alias
 eNOS-interacting protein 
Gene Name
 NOSIP 
Gene Synonyms/Alias
 CGI-25 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
6**MTRHGKNCTAGAVubiquitination[1]
20VYTYHEKKKDTAASGubiquitination[1, 2]
21YTYHEKKKDTAASGYubiquitination[1, 2]
41RLSRDAVKDFDCCCLubiquitination[1]
79EYILHQKKEIARQMKubiquitination[1]
117HVRGFLEKESAIVSRubiquitination[1, 2]
132PLNPFTAKALSGTSPubiquitination[1]
153PSVGPPSKDKDKVLPubiquitination[1, 3]
155VGPPSKDKDKVLPSFubiquitination[1]
157PPSKDKDKVLPSFWIubiquitination[1, 4, 5]
175TPEAKATKLEKPSRTubiquitination[1]
178AKATKLEKPSRTVTCubiquitination[1]
190VTCPMSGKPLRMSDLubiquitination[1]
253VTLECVEKLIRKDMVubiquitination[1, 6]
267VDPVTGDKLTDRDIIubiquitination[1, 6]
289GFAGSGVKLQAEKSRubiquitination[1, 2, 6]
294GVKLQAEKSRPVMQAacetylation[6]
294GVKLQAEKSRPVMQAubiquitination[1, 2, 6]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity. 
Sequence Annotation
 REGION 55 75 U-box-like.
 MOTIF 78 101 Nuclear localization signal.  
Keyword
 Complete proteome; Cytoplasm; Nucleus; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 301 AA 
Protein Sequence
MTRHGKNCTA GAVYTYHEKK KDTAASGYGT QNIRLSRDAV KDFDCCCLSL QPCHDPVVTP 60
DGYLYEREAI LEYILHQKKE IARQMKAYEK QRGTRREEQK ELQRAASQDH VRGFLEKESA 120
IVSRPLNPFT AKALSGTSPD DVQPGPSVGP PSKDKDKVLP SFWIPSLTPE AKATKLEKPS 180
RTVTCPMSGK PLRMSDLTPV HFTPLDSSVD RVGLITRSER YVCAVTRDSL SNATPCAVLR 240
PSGAVVTLEC VEKLIRKDMV DPVTGDKLTD RDIIVLQRGG TGFAGSGVKL QAEKSRPVMQ 300
A 301 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0000139; C:Golgi membrane; TAS:Reactome.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0051001; P:negative regulation of nitric-oxide synthase activity; IDA:UniProtKB.
 GO:0046209; P:nitric oxide metabolic process; TAS:Reactome.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 
Interpro
 IPR016818; Nitric_oxide_synth-interacting.
 IPR013083; Znf_RING/FYVE/PHD. 
Pfam
  
SMART
  
PROSITE
  
PRINTS