UniProt Accession

 NEDD8_MOUSE; P29595 

Genbank Protein ID

 D10918; BC004625 

Genbank Nucleotide ID

 BAA01719.1; AAH04625.1 

Protein Name

 NEDD8 

Protein Synonyms/Alias

 Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; NEDD-8; Ubiquitin-like protein Nedd8 

Gene Name

 Nedd8 

Gene Synonyms/Alias

 Nedd-8 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
6	**MLIKVKTLTGKEI	ubiquitination	[1]
11	KVKTLTGKEIEIDIE	ubiquitination	[1]
22	IDIEPTDKVERIKER	acetylation	[2]
22	IDIEPTDKVERIKER	ubiquitination	[1]
33	IKERVEEKEGIPPQQ	ubiquitination	[1]
48	QRLIYSGKQMNDEKT	acetylation	[2, 3, 4]
48	QRLIYSGKQMNDEKT	ubiquitination	[1]
54	GKQMNDEKTAADYKI	acetylation	[4]
54	GKQMNDEKTAADYKI	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C- APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. 

Sequence Annotation

 REGION 70 72 Interaction with UBE1C (By similarity).
 CROSSLNK 76 76 Glycyl lysine isopeptide (Gly-Lys)  

Keyword

 Complete proteome; Isopeptide bond; Nucleus; Reference proteome; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 81 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; IDA:MGI.
 GO:0008104; P:protein localization; IDA:MGI.
 GO:0045116; P:protein neddylation; ISA:MGI.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IDA:MGI.
 GO:0014070; P:response to organic cyclic compound; IEA:Compara. 

Interpro

 IPR000626; Ubiquitin.
 IPR019954; Ubiquitin_CS.
 IPR019956; Ubiquitin_subgr.
 IPR019955; Ubiquitin_supergroup. 

Pfam

 PF00240; ubiquitin 

SMART

 SM00213; UBQ 

PROSITE

 PS00299; UBIQUITIN_1
 PS50053; UBIQUITIN_2 

PRINTS

 PR00348; UBIQUITIN.