UniProt Accession

 UBC1_YEAST; P21734; D6VSF9 

Genbank Protein ID

 X56402; Z46727; AY557675; BK006938 

Genbank Nucleotide ID

 CAA39812.1; CAA86682.1; AAS56001.1; DAA12019.1 

Protein Name

 Ubiquitin-conjugating enzyme E2 1 

Protein Synonyms/Alias

 Ubiquitin carrier protein; Ubiquitin-conjugating enzyme E2-24 kDa; Ubiquitin-protein ligase 

Gene Name

 UBC1 

Gene Synonyms/Alias

 YDR177W; YD9395.10 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
93	AICLDILKNAWSPVI	ubiquitination	[1, 2]
139	RDRESFNKTAALWTR	ubiquitination	[2]
196	VLRRLGVKSLDPNDN	ubiquitination	[2]

Reference

 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in degradation of misfolded or regulated proteins localized in the endoplasmic reticulum (ER) lumen or membrane via the ubiquitin-proteasome system. Cognate E2 conjugating enzyme for the HRD1 ubiquitin ligase complex, which is part of the ERAD-L and ERAD-M pathways responsible for the rapid degradation of soluble lumenal and membrane proteins with misfolded lumenal domains (ERAD-L), or ER-membrane proteins with misfolded transmembrane domains (ERAD-M). 

Sequence Annotation

 ACT_SITE 88 88 Glycyl thioester intermediate.  

Keyword

 3D-structure; ATP-binding; Complete proteome; Ligase; Nucleotide-binding; Reference proteome; Stress response; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 215 AA 

Protein Sequence

Gene Ontology

 GO:0000502; C:proteasome complex; IPI:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004842; F:ubiquitin-protein ligase activity; IDA:SGD.
 GO:0006897; P:endocytosis; TAS:SGD.
 GO:0030433; P:ER-associated protein catabolic process; IMP:SGD.
 GO:0006513; P:protein monoubiquitination; TAS:SGD.
 GO:0000209; P:protein polyubiquitination; TAS:SGD.
 GO:0042787; P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process; IMP:SGD.
 GO:0006950; P:response to stress; IEA:UniProtKB-KW.
 GO:0009847; P:spore germination; TAS:SGD.
 GO:0016050; P:vesicle organization; IMP:SGD. 

Interpro

 IPR009060; UBA-like.
 IPR015368; UBA_C_fun.
 IPR000608; UBQ-conjugat_E2.
 IPR023313; UBQ-conjugating_AS.
 IPR016135; UBQ-conjugating_enzyme/RWD. 

Pfam

 PF09288; UBA_3
 PF00179; UQ_con 

SMART

  

PROSITE

 PS00183; UBIQUITIN_CONJUGAT_1
 PS50127; UBIQUITIN_CONJUGAT_2 

PRINTS