UniProt Accession

 MIPEP_MOUSE; A6H611; Q2YD79; Q3UJJ3; Q80VA3 

Genbank Protein ID

 AK146428; BC049871; BC110358; BC145711 

Genbank Nucleotide ID

 BAE27162.1; AAH49871.1; AAI10359.1; AAI45712.1 

Protein Name

 Mitochondrial intermediate peptidase 

Protein Synonyms/Alias

 MIP 

Gene Name

 Mipep 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
124	ADLADFVKIGHPDPA	acetylation	[1, 2]
168	QRLLGDKKLMESLDA	acetylation	[3]

Reference

 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [3] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Cleaves proteins, imported into the mitochondrion, to their mature size (By similarity). 

Sequence Annotation

 ACT_SITE 494 494 By similarity.
 METAL 493 493 Zinc; catalytic (By similarity).
 METAL 497 497 Zinc; catalytic (By similarity).
 METAL 500 500 Zinc; catalytic (By similarity).
 MOD_RES 124 124 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Calcium; Cobalt; Complete proteome; Hydrolase; Iron; Magnesium; Manganese; Metal-binding; Metalloprotease; Mitochondrion; Protease; Reference proteome; Transit peptide; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 711 AA 

Protein Sequence

Gene Ontology

 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 

Interpro

 IPR024079; MetalloPept_cat_dom.
 IPR024077; Neurolysin/TOP_dom2.
 IPR024080; Neurolysin/TOP_N.
 IPR001567; Pept_M3A_M3B. 

Pfam

 PF01432; Peptidase_M3 

SMART

  

PROSITE

 PS00142; ZINC_PROTEASE 

PRINTS