UniProt Accession

 KFA_MOUSE; Q8K4H1; B1AQK9; Q80XP3; Q8R1K6 

Genbank Protein ID

 AF399717; AY099479; AL591433; BC024452; BC043309; BC066780 

Genbank Nucleotide ID

 AAM62284.1; AAM44406.1; CAM19096.1; AAH24452.1; AAH43309.1; AAH66780.1 

Protein Name

 Kynurenine formamidase 

Protein Synonyms/Alias

 KFA; KFase; Arylformamidase; N-formylkynurenine formamidase; FKF 

Gene Name

 Afmid 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
23	LSSEELEKQYSPSRW	ubiquitination	[1]
256	PEFHRQSKEFYETLL	ubiquitination	[1]
299	VLTQIILKTVFQKL*	acetylation	[2, 3, 4]
299	VLTQIILKTVFQKL*	succinylation	[3]
299	VLTQIILKTVFQKL*	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 

Functional Description

 Catalyzes the hydrolysis of N-formyl-L-kynurenine to L- kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites. 

Sequence Annotation

 MOTIF 93 97 HGGXW.
 ACT_SITE 162 162 Nucleophile (Probable).
 ACT_SITE 247 247 Probable.
 ACT_SITE 279 279 Probable.  

Keyword

 Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Nucleus; Reference proteome; Tryptophan catabolism. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 305 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0004061; F:arylformamidase activity; IEA:HAMAP.
 GO:0034354; P:de novo NAD biosynthetic process from tryptophan; IEA:HAMAP.
 GO:0019441; P:tryptophan catabolic process to kynurenine; IDA:MGI. 

Interpro

 IPR013094; AB_hydrolase_3.
 IPR027519; KFase. 

Pfam

 PF07859; Abhydrolase_3 

SMART

  

PROSITE

  

PRINTS