CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-036399
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Adenosylhomocysteinase 
Protein Synonyms/Alias
  
Gene Name
 AHCYL2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
246HRLYQLSKAGKLCVPubiquitination[1, 2]
249YQLSKAGKLCVPAMNubiquitination[1]
263NVNDSVTKQKFDNLYubiquitination[1, 3]
265NDSVTKQKFDNLYCCubiquitination[1, 2]
281ESILDGLKRTTDMMFubiquitination[4]
411HVIWPDGKRIVLLAEubiquitination[1, 4]
457NAPEGRYKQDVYLLPubiquitination[1]
466DVYLLPKKMDEYVASubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
  
Sequence Annotation
 BINDING 134 134 Substrate (By similarity).
 BINDING 208 208 Substrate (By similarity).
 BINDING 233 233 Substrate (By similarity).
 BINDING 263 263 Substrate (By similarity).
 BINDING 267 267 Substrate (By similarity).  
Keyword
 Complete proteome; Hydrolase; NAD; One-carbon metabolism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 509 AA 
Protein Sequence
MEKWDGNEGT SAFHMPEWMQ IQFADQKQEF NKRPTKIGRR SLSRSISQSS TDSYSSAASY 60
TDSSDDETSP RDKQQKNSKG SSDFCVKNIK QAEFGRREIE IAEQEMPALM ALRKRAQGEK 120
PLAGAKIVGC THITAQTAVL METLGALGAQ CRWAACNIYS TLNEVAAALA ESGFPVFAWK 180
GESEDDFWWC IDRCVNVEGW QPNMILDDGG DLTHWIYKKY PNMFKKIKGI VEESVTGVHR 240
LYQLSKAGKL CVPAMNVNDS VTKQKFDNLY CCRESILDGL KRTTDMMFGG KQVVVCGYGE 300
VGKGCCAALK AMGSIVYVTE IDPICALQAC MDGFRLVKLN EVIRQVDIVI TCTGNKNVVT 360
REHLDRMKNS CIVCNMGHSN TEIDVASLRT PELTWERVRS QVDHVIWPDG KRIVLLAEGR 420
LLNLSCSTVP TFVLSITATT QALALIELYN APEGRYKQDV YLLPKKMDEY VASLHLPTFD 480
AHLTELTDEQ AKYLGLNKNG PFKPNYYRY 509 
Gene Ontology
 GO:0004013; F:adenosylhomocysteinase activity; IEA:EC.
 GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW. 
Interpro
 IPR000043; Adenosylhomocysteinase.
 IPR015878; Ado_hCys_hydrolase_NAD-bd.
 IPR016040; NAD(P)-bd_dom.
 IPR020082; S-Ado-L-homoCys_hydrolase_CS. 
Pfam
 PF05221; AdoHcyase
 PF00670; AdoHcyase_NAD 
SMART
 SM00996; AdoHcyase
 SM00997; AdoHcyase_NAD 
PROSITE
 PS00738; ADOHCYASE_1
 PS00739; ADOHCYASE_2 
PRINTS