UniProt Accession

 MOV10_HUMAN; Q9HCE1; Q5JR03; Q8TEF0; Q9BSY3; Q9BUJ9 

Genbank Protein ID

 AB046851; AK074174; AL833353; AL603832; BC002548; BC004499; BC009312 

Genbank Nucleotide ID

 BAB13457.1; BAB85000.1; CAI14055.1; AAH02548.1; AAH04499.2; AAH09312.1 

Protein Name

 Putative helicase MOV-10 

Protein Synonyms/Alias

 Moloney leukemia virus 10 protein 

Gene Name

 MOV10 

Gene Synonyms/Alias

 KIAA1631 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
71	ANLAYVTKTRVRFFR	ubiquitination	[1, 2]
90	ADVRFPEKRRMKLGS	ubiquitination	[2]
94	FPEKRRMKLGSDISK	ubiquitination	[2]
109	HHKSLLAKIFYDRAE	ubiquitination	[1, 2, 3, 4, 5]
121	RAEYLHGKHGVDVEV	ubiquitination	[2]
148	IRLDLNRKEVLTLRL	acetylation	[6]
148	IRLDLNRKEVLTLRL	ubiquitination	[2]
251	SPLAAQLKPMTPFKR	ubiquitination	[2]
257	LKPMTPFKRTRITGN	ubiquitination	[2]
318	TSIFTAPKEIAEIKA	ubiquitination	[1]
324	PKEIAEIKAQLETAL	ubiquitination	[2]
339	KWRNYEVKLRLLLHL	ubiquitination	[2]
414	QEDPITYKGFVHKVE	ubiquitination	[3, 5]
489	PLLPSDVKLKLYDRS	ubiquitination	[2, 3, 5]
491	LPSDVKLKLYDRSLE	ubiquitination	[2]
530	FGPPGTGKTVTLVEA	ubiquitination	[2]
539	VTLVEAIKQVVKHLP	ubiquitination	[2]
547	QVVKHLPKAHILACA	ubiquitination	[2]
593	RMVPEDIKPCCNWDA	ubiquitination	[2]
602	CCNWDAKKGEYVFPA	ubiquitination	[2]
695	LRSPLTQKHGLGYSL	ubiquitination	[2, 3, 5]
714	LTYNSLYKKGPDGYD	ubiquitination	[2]
715	TYNSLYKKGPDGYDP	ubiquitination	[2]
727	YDPQFITKLLRNYRS	ubiquitination	[1, 2]
784	IFHGVMGKDEREGNS	ubiquitination	[2]
816	LLLAPSSKKGKARLS	ubiquitination	[7]
817	LLAPSSKKGKARLSP	ubiquitination	[2]
846	KIRYCITKLDRELRG	ubiquitination	[2]
858	LRGLDDIKDLKVGSV	ubiquitination	[2, 3, 5]
861	LDDIKDLKVGSVEEF	ubiquitination	[2]
967	NLLQGLSKLSPSTSG	ubiquitination	[2, 3, 5]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 Probable RNA helicase. Required for RNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA- mediated cleavage of complementary mRNAs by RISC. Also required for RNA-directed transcription and replication of the human hepatitis delta virus (HDV). Interacts with small capped HDV RNAs derived from genomic hairpin structures that mark the initiation sites of RNA-dependent HDV RNA transcription. 

Sequence Annotation

 NP_BIND 524 531 ATP (By similarity).
 REGION 921 965 Interaction with AGO2 and APOBEC3G.
 MOTIF 645 648 DEAG box.
 MOD_RES 148 148 N6-acetyllysine.
 MOD_RES 160 160 Phosphothreonine.
 MOD_RES 254 254 Phosphothreonine.
 MOD_RES 969 969 Phosphoserine.  

Keyword

 Acetylation; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Helicase; Hydrolase; Nucleotide-binding; Phosphoprotein; Reference proteome; RNA-binding; RNA-mediated gene silencing; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1003 AA 

Protein Sequence

Gene Ontology

 GO:0000932; C:cytoplasmic mRNA processing body; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
 GO:0008543; P:fibroblast growth factor receptor signaling pathway; TAS:Reactome.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0035279; P:mRNA cleavage involved in gene silencing by miRNA; IDA:UniProtKB.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0007219; P:Notch signaling pathway; TAS:Reactome.
 GO:0048015; P:phosphatidylinositol-mediated signaling; TAS:Reactome.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR026122; MOV-10/Armitage.
 IPR027417; P-loop_NTPase. 

Pfam

  

SMART

  

PROSITE

  

PRINTS