UniProt Accession

 MNMA_ECOLI; P25745; P75964 

Genbank Protein ID

 U00096; AP009048; X59307; M74924 

Genbank Nucleotide ID

 AAC74217.2; BAA35955.1; CAA41994.1 

Protein Name

 tRNA-specific 2-thiouridylase MnmA 

Protein Synonyms/Alias

  

Gene Name

 mnmA 

Gene Synonyms/Alias

 asuE; trmU; ycfB; b1133; JW1119 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
104	NPDILCNKEIKFKAF	acetylation	[1]
107	ILCNKEIKFKAFLEF	acetylation	[1]
149	LRGLDSNKDQSYFLY	acetylation	[1, 2]
175	FPVGELEKPQVRKIA	acetylation	[1]
252	GLGIGGTKEGTEEPW	acetylation	[1]
264	EPWYVVDKDVENNIL	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

 Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA(Lys), tRNA(Glu) and tRNA(Gln), leading to the formation of s(2)U34, the first step of tRNA-mnm(5)s(2)U34 synthesis. Sulfur is provided by IscS, via a sulfur-relay system. Binds ATP and its substrate tRNAs. 

Sequence Annotation

 NP_BIND 11 18 ATP.
 REGION 97 99 Interaction with target base in tRNA.
 REGION 149 151 Interaction with tRNA.
 REGION 243 252 Interaction with tRNA.
 REGION 311 312 Interaction with tRNA.
 ACT_SITE 102 102 Nucleophile.
 ACT_SITE 199 199 Cysteine persulfide intermediate.
 BINDING 37 37 ATP; via amide nitrogen and carbonyl
 BINDING 127 127 ATP; via amide nitrogen.
 DISULFID 102 199 Alternate.  

Keyword

 3D-structure; ATP-binding; Complete proteome; Cytoplasm; Disulfide bond; Nucleotide-binding; Reference proteome; RNA-binding; Transferase; tRNA processing; tRNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 368 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016783; F:sulfurtransferase activity; IDA:EcoCyc.
 GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
 GO:0002143; P:tRNA wobble position uridine thiolation; IDA:EcoCyc. 

Interpro

 IPR023382; Adenine_a_hdrlase_dom.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR004506; tRNA-specific_2-thiouridylase. 

Pfam

 PF03054; tRNA_Me_trans 

SMART

  

PROSITE

  

PRINTS