| Tag | Content |
|---|
| CPLM ID | CPLM-002060 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Site-specific recombinase Flp |
Protein Synonyms/Alias | FLP; Protein Able |
Gene Name | FLP1 |
Gene Synonyms/Alias | R0010W |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 269 | TGNSSSNKQEYQLLK | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] |
Functional Description | Part of the plasmid amplification system, which corrects any decrease in copy number caused by a rare missegregation event. Catalyzes the recombination between the large inverted repetitions of the 2-micron plasmid during plasmid replication. This recombination event changes the direction of one of the two replication forks in the bidirectionally replicating molecule, effectively resulting in multiple rounds of replication from a single initiation event. Binds specifically to the FLP recognition target (FRT) site where it induces DNA to bend. Three types of bend exist. Type I is approximately 60 degrees and results from 1 FLP molecule binding to 1 symmetry element. Type II is >144 degrees and results from FLP molecules binding to symmetry elements a and b. Type III is approximately 65 degrees and results from FLP molecules binding to symmetry elements b and c. |
Sequence Annotation | ACT_SITE 343 343 O-(3'-phospho-DNA)-tyrosine intermediate. |
Keyword | 3D-structure; Complete proteome; Direct protein sequencing; DNA integration; DNA recombination; DNA-binding; Plasmid; Reference proteome. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 423 AA |
Protein Sequence | MPQFGILCKT PPKVLVRQFV ERFERPSGEK IALCAAELTY LCWMITHNGT AIKRATFMSY 60
NTIISNSLSF DIVNKSLQFK YKTQKATILE ASLKKLIPAW EFTIIPYYGQ KHQSDITDIV 120
SSLQLQFESS EEADKGNSHS KKMLKALLSE GESIWEITEK ILNSFEYTSR FTKTKTLYQF 180
LFLATFINCG RFSDIKNVDP KSFKLVQNKY LGVIIQCLVT ETKTSVSRHI YFFSARGRID 240
PLVYLDEFLR NSEPVLKRVN RTGNSSSNKQ EYQLLKDNLV RSYNKALKKN APYSIFAIKN 300
GPKSHIGRHL MTSFLSMKGL TELTNVVGNW SDKRASAVAR TTYTHQITAI PDHYFALVSR 360
YYAYDPISKE MIALKDETNP IEEWQHIEQL KGSAEGSIRY PAWNGIISQE VLDYLSSYIN 420
RRI 423 |
Gene Ontology | GO:0008301; F:DNA binding, bending; IMP:SGD. GO:0003690; F:double-stranded DNA binding; IDA:SGD. GO:0003697; F:single-stranded DNA binding; IDA:SGD. GO:0009009; F:site-specific recombinase activity; IDA:SGD. GO:0015074; P:DNA integration; IEA:UniProtKB-KW. GO:0042150; P:plasmid recombination; IDA:SGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |