CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-038310
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Intersectin-1 
Protein Synonyms/Alias
  
Gene Name
 Itsn1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
475VVLKARRKTLEFELEubiquitination[1]
574LKRALEAKELARQQLubiquitination[1]
607DVFNNQLKELREIHSubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome; SH3 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1719 AA 
Protein Sequence
MAQFPTPFGG SLDVWAITVE ERAKHDQQFL SLKPIAGFIT GDQARNFFFQ SGLPQPVLAQ 60
IWALADMNND GRMDQVEFSI AMKLIKLKLQ GYQLPSTLPP VMKQQPVAIS SAPAFGIGGI 120
ASMPPLTAVA PVPMGSIPVV GMSPPLVSSV PPAAVPPLAN GAPPVIQPLP AFAHPAATLP 180
KSSSFSRSGP GSQLNTKLQK AQSFDVASAP PAAEWAVPQS SRLKYRQLFN SHDKTMSGHL 240
TGPQARTILM QSSLPQAQLA SIWNLSDIDQ DGKLTAEEFI LAMHLIDVAM SGQPLPPVLP 300
PEYIPPSFRR VRSGSGMSVI SSSSVDQRLP EEPSSEDEQQ PEKKLPVTFE DKKRENFERG 360
SVELEKRRQA LLEQQRKEQE RLAQLERAEQ ERKERERQEQ ERKRQLELEK QLEKQRELER 420
QREEERRKEI ERREAAKREL ERQRQLEWER NRRQELLNQR NKEQEGTVVL KARRKTLEFE 480
LEALNDKKHQ LEGKLQDIRC RLATQRQEIE STNKSRELRI AEITHLQQQL QESQQMLGRL 540
IPEKQILSDQ LKQVQQNSLH RDSLLTLKRA LEAKELARQQ LREQLDEVER ETRSKLQEID 600
VFNNQLKELR EIHSKQQLQK QRSLEAARLK QKEQERKSLE LEKQKEDAQR RVQERDKQWL 660
EHVQQEEQPR PRKPHEEDRL KREDSVRKKE AEERAKPEMQ DKQSRLFHPH QEPAKLATQA 720
PWSTTEKGPL TISAQESVKV VYYRALYPFE SRSHDEITIQ PGDIVMVKGE WVDESQTGEP 780
GWLGGELKGK TGWFPANYAE KIPENEVPTP AKPVTDLTSA PAPKLALRET PAPLPVTSSE 840
PSTTPNNWAD FSSTWPSSSN EKPETDNWDT WAAQPSLTVP SAGQLRQRSA FTPATATGSS 900
PSPVLGQGEK VEGLQAQALY PWRAKKDNHL NFNKSDVITV LEQQDMWWFG EVQGQKGWFP 960
KSYVKLISGP VRKSTSIDTG PTESPASLKR VASPAAKPAI PGEEFIAMYT YESSEQGDLT 1020
FQQGDVIVVT KKDGDWWTGT VGDKSGVFPS NYVRLKDSEG SGTAGKTGSL GKKPEIAQVI 1080
ASYAATGPEQ LTLAPGQLIL IRKKNPGGWW EGELQARGKK RQIGWFPANY VKLLSPGTSK 1140
ITPTELPKTA VQPAVCQVIG MYDYTAQNDD ELAFSKGQII NVLNKEDPDW WKGEVSGQVG 1200
LFPSNYVKLT TDMDPSQQWC SDLHLLDMLT PTERKRQGYI HELIVTEENY VNDLQLVTEI 1260
FQKPLTESEL LTEKEVAMIF VNWKELIMCN IKLLKALRVR KKMSGEKMPV KMIGDILSAQ 1320
LPHMQPYIRF CSCQLNGAAL IQQKTDEAPD FKEFVKRLAM DPRCKGMPLS SFILKPMQRV 1380
TRYPLIIKNI LENTPENHPD HSHLKHALEK AEELCSQVNE GVREKENSDR LEWIQAHVQC 1440
EGLSEQLVFN SVTNCLGPRK FLHSGKLYKA KSNKELYGFL FNDFLLLTQI TKPLGSSGTD 1500
KVFSPKSNLQ YKMYKTPIFL NEVLVKLPTD PSGDEPIFHI SHIDRVYTLR AESINERTAW 1560
VQKIKAASEL YIETEKKKRE KAYLVRSQRA TGIGRLMVNV VEGIELKPCR SHGKSNPYCE 1620
VTMGSQCHIT KTIQDTLNPK WNSNCQFFIR DLEQEVLCIT VFERDQFSPD DFLGRTEIRV 1680
ADIKKDQGSK GPVTKCLLLH EVPTGEIVVR LDLQLFDEP 1719 
Gene Ontology
 GO:0030139; C:endocytic vesicle; IDA:MGI.
 GO:0030027; C:lamellipodium; IDA:MGI.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0019209; F:kinase activator activity; IDA:MGI.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0005089; F:Rho guanyl-nucleotide exchange factor activity; IEA:InterPro.
 GO:0006897; P:endocytosis; IEA:InterPro.
 GO:0043524; P:negative regulation of neuron apoptotic process; IMP:MGI.
 GO:0051897; P:positive regulation of protein kinase B signaling cascade; IDA:MGI.
 GO:0035023; P:regulation of Rho protein signal transduction; IEA:InterPro.
 GO:0007264; P:small GTPase mediated signal transduction; IDA:MGI. 
Interpro
 IPR000008; C2_Ca-dep.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR018029; C2_membr_targeting.
 IPR000219; DH-domain.
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR000261; EPS15_homology.
 IPR001331; GDS_CDC24_CS.
 IPR026813; ITSN1.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR011511; SH3_2.
 IPR001452; SH3_domain. 
Pfam
 PF00168; C2
 PF00169; PH
 PF00621; RhoGEF
 PF00018; SH3_1
 PF07653; SH3_2 
SMART
 SM00239; C2
 SM00054; EFh
 SM00027; EH
 SM00233; PH
 SM00325; RhoGEF
 SM00326; SH3 
PROSITE
 PS50004; C2
 PS00741; DH_1
 PS50010; DH_2
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS50031; EH
 PS50003; PH_DOMAIN
 PS50002; SH3 
PRINTS
 PR00452; SH3DOMAIN.