UniProt Accession

 NUP49_YEAST; Q02199; D6VTY0 

Genbank Protein ID

 X68109; Z15040; X84705; Z72694; BK006941 

Genbank Nucleotide ID

 CAA48229.1; CAA78758.1; CAA59181.1; CAA96884.1; DAA07941.1 

Protein Name

 Nucleoporin NUP49/NSP49 

Protein Synonyms/Alias

 Nuclear pore protein NUP49/NSP49 

Gene Name

 NUP49 

Gene Synonyms/Alias

 NSP49; YGL172W; G1648 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
327	SATSQYLKQDLKKIS	ubiquitination	[1]
371	ISSNDLDKFFQKKIH	acetylation	[2]
382	KKIHLYEKKLEDYCR	acetylation	[2]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). NUP49 plays an important role in several nuclear transport pathways including poly(A)+ RNA, tRNA, and pre-ribosome transport. 

Sequence Annotation

 REPEAT 2 3 FG 1.
 REPEAT 14 17 GLFG 1.
 REPEAT 33 34 FG 2.
 REPEAT 48 51 GLFG 2.
 REPEAT 65 66 FG 3.
 REPEAT 77 78 FG 4.
 REPEAT 86 89 GLFG 3.
 REPEAT 101 104 GLFG 4.
 REPEAT 113 116 SLFG 1.
 REPEAT 125 128 GLFG 5.
 REPEAT 148 151 GLFG 6.
 REPEAT 159 162 SLFG 2.
 REPEAT 175 178 GLFG 7; approximate.
 REPEAT 185 188 SLFG 3.
 REPEAT 199 202 GLFG 8.
 REPEAT 210 213 SLFG 4.
 REPEAT 233 236 GLFG 9.  

Keyword

 Coiled coil; Complete proteome; Membrane; mRNA transport; Nuclear pore complex; Nucleus; Protein transport; Reference proteome; Repeat; Translocation; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 472 AA 

Protein Sequence

Gene Ontology

 GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
 GO:0044613; C:nuclear pore central transport channel; IDA:SGD.
 GO:0005487; F:nucleocytoplasmic transporter activity; IGI:SGD.
 GO:0017056; F:structural constituent of nuclear pore; IDA:SGD.
 GO:0016973; P:poly(A)+ mRNA export from nucleus; IMP:SGD.
 GO:0006606; P:protein import into nucleus; IMP:SGD.
 GO:0000055; P:ribosomal large subunit export from nucleus; IMP:SGD.
 GO:0006409; P:tRNA export from nucleus; IMP:SGD. 

Interpro

 IPR025574; Nucleoporin_FG_rpt. 

Pfam

 PF13634; Nucleoporin_FG 

SMART

  

PROSITE

  

PRINTS