UniProt Accession

 CIAO1_HUMAN; O76071; A0MNN9; Q53FM5 

Genbank Protein ID

 U63810; EF011618; AC004020; CR456802; AK223257; BC001395; BC032812 

Genbank Nucleotide ID

 AAC24948.1; ABK41108.1; AAC23493.1; CAG33083.1; BAD96977.1; AAH01395.1; AAH32812.1 

Protein Name

 Probable cytosolic iron-sulfur protein assembly protein CIAO1 

Protein Synonyms/Alias

 WD repeat-containing protein 39 

Gene Name

 CIAO1 

Gene Synonyms/Alias

 WDR39 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
2	******MKDSLVLLG	ubiquitination	[1, 2, 3, 4, 5]
53	EGDSWICKSVLSEGH	ubiquitination	[1, 3, 4, 5]
90	ATTCIWKKNQDDFEC	ubiquitination	[3]
109	EGHENEVKSVAWAPS	ubiquitination	[3, 5, 6]
242	SGSDPSWKCICTLSG	ubiquitination	[6]
332	DGEVAFWKYQRPEGL	ubiquitination	[3, 6]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 

Functional Description

 Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. Seems to specifically modulate the transactivation activity of WT1. As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation. 

Sequence Annotation

 REPEAT 14 53 WD 1.
 REPEAT 59 98 WD 2.
 REPEAT 103 142 WD 3.
 REPEAT 148 187 WD 4.
 REPEAT 192 231 WD 5.
 REPEAT 250 289 WD 6.
 REPEAT 301 339 WD 7.  

Keyword

 3D-structure; Chromosome partition; Complete proteome; Reference proteome; Repeat; WD repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 339 AA 

Protein Sequence

Gene Ontology

 GO:0097361; C:CIA complex; IDA:UniProtKB.
 GO:0071817; C:MMXD complex; IDA:UniProtKB.
 GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
 GO:0016226; P:iron-sulfur cluster assembly; IGI:UniProtKB.
 GO:0008284; P:positive regulation of cell proliferation; TAS:ProtInc.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; TAS:ProtInc.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 

Interpro

 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 

Pfam

 PF00400; WD40 

SMART

 SM00320; WD40 

PROSITE

 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 

PRINTS