CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-041298
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NOL1/NOP2/Sun domain family, member 2, isoform CRA_b 
Protein Synonyms/Alias
 tRNA (cytosine(34)-C(5))-methyltransferase 
Gene Name
 NSUN2 
Gene Synonyms/Alias
 hCG_18368 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
21QIDVDGRKEILFYDRubiquitination[1]
43SGDGTMRKNIDVWKKubiquitination[1]
50KNIDVWKKWTTLNSLubiquitination[1, 2]
120SNELPGLKWMPGITQubiquitination[2, 3, 4]
129MPGITQWKVMTKDGQubiquitination[1, 4, 5]
133TQWKVMTKDGQWFTDubiquitination[2, 4]
201KSSMPWNKRQPKLQGubiquitination[1]
228PADLTEGKPTDPSKLacetylation[6]
273CGPPPSKKMKLFGFKubiquitination[1]
275PPPSKKMKLFGFKEDubiquitination[5]
329RQLYMVSKELRNVLLubiquitination[1]
341VLLNNSEKMKVINTGubiquitination[1, 2, 3, 4, 7]
343LNNSEKMKVINTGIKubiquitination[1, 3]
350KVINTGIKVWCRNNSacetylation[6, 7, 8]
350KVINTGIKVWCRNNSubiquitination[1]
392TVSMEDVKILLTQENubiquitination[3]
404QENPFFRKLSSETYSubiquitination[1, 2, 3, 7]
414SETYSQAKDLAKGSIubiquitination[1, 2, 3, 4, 5, 7]
418SQAKDLAKGSIVLKYubiquitination[1, 2, 3, 4, 5, 7]
424AKGSIVLKYEPDSANubiquitination[2, 4, 7]
456SIRTFVPKNERLHYLubiquitination[1, 5, 7]
475LEVLGEKKKEGVILTubiquitination[1, 2, 4, 7]
476EVLGEKKKEGVILTNubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [8] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 531 AA 
Protein Sequence
MVVNHDASSI PRLQIDVDGR KEILFYDRIL CDVPCSGDGT MRKNIDVWKK WTTLNSLQLH 60
GLQLRIATRG AEQLAEGGRM VYSTCSLNPI EDEAVIASLL EKSEGALELA DVSNELPGLK 120
WMPGITQWKV MTKDGQWFTD WDAVPHSRHT QIRPTMFPPK DPEKLQAMHL ERCLRILPHH 180
QNTGGFFVAV LVKKSSMPWN KRQPKLQGKS AETRESTQLS PADLTEGKPT DPSKLESPSF 240
TGTGDTEIAH ATEDLENNGS KKDGVCGPPP SKKMKLFGFK EDPFVFIPED DPLFPPIEKF 300
YALDPSFPRM NLLTRTTEGK KRQLYMVSKE LRNVLLNNSE KMKVINTGIK VWCRNNSGEE 360
FDCAFRLAQE GIYTLYPFIN SRIITVSMED VKILLTQENP FFRKLSSETY SQAKDLAKGS 420
IVLKYEPDSA NPDALQCPIV LCGWRGKASI RTFVPKNERL HYLRMMGLEV LGEKKKEGVI 480
LTNESAASTG QPDNDVTEGQ RAGEPNSPDA EEANSPDVTA GCDPAGVHPP R 531 
Gene Ontology
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0016428; F:tRNA (cytosine-5-)-methyltransferase activity; IEA:InterPro. 
Interpro
 IPR001678; Fmu/NOL1/Nop2p.
 IPR023267; RCMT.
 IPR023270; RCMT_NCL1. 
Pfam
 PF01189; Nol1_Nop2_Fmu 
SMART
  
PROSITE
  
PRINTS
 PR02008; RCMTFAMILY.
 PR02011; RCMTNCL1.