UniProt Accession

 KTHY_HUMAN; P23919; Q6FGX1; Q9BUX4 

Genbank Protein ID

 X54729; L16991; CR541986; BC001827 

Genbank Nucleotide ID

 CAA38528.1; AAA21719.1; CAG46783.1; AAH01827.1 

Protein Name

 Thymidylate kinase 

Protein Synonyms/Alias

 dTMP kinase 

Gene Name

 DTYMK 

Gene Synonyms/Alias

 CDC8; TMPK; TYMK 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
51	ERSTEIGKLLSSYLQ	ubiquitination	[1, 2, 3]
169	RCFHQLMKDTTLNWK	acetylation	[4]
182	WKMVDASKSIEAVHE	ubiquitination	[5]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Catalyzes the conversion of dTMP to dTDP. 

Sequence Annotation

 NP_BIND 13 20 ATP (Probable).
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 169 169 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; ATP-binding; Complete proteome; Kinase; Nucleotide biosynthesis; Nucleotide-binding; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 212 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005758; C:mitochondrial intermembrane space; IEA:Compara.
 GO:0005759; C:mitochondrial matrix; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0050145; F:nucleoside phosphate kinase activity; EXP:Reactome.
 GO:0004798; F:thymidylate kinase activity; IDA:MGI.
 GO:0007049; P:cell cycle; TAS:ProtInc.
 GO:0008283; P:cell proliferation; TAS:ProtInc.
 GO:0071363; P:cellular response to growth factor stimulus; IEA:Compara.
 GO:0006233; P:dTDP biosynthetic process; IEA:Compara.
 GO:0006235; P:dTTP biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0045445; P:myoblast differentiation; IEA:Compara.
 GO:0015949; P:nucleobase-containing small molecule interconversion; TAS:Reactome.
 GO:0046686; P:response to cadmium ion; IEA:Compara.
 GO:0043627; P:response to estrogen stimulus; IEA:Compara. 

Interpro

 IPR027417; P-loop_NTPase.
 IPR018095; Thymidylate_kin_CS.
 IPR018094; Thymidylate_kinase. 

Pfam

  

SMART

  

PROSITE

 PS01331; THYMIDYLATE_KINASE 

PRINTS