CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009514
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase 
Protein Synonyms/Alias
 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase; Protein GcpE; Protein E 
Gene Name
 ispG 
Gene Synonyms/Alias
 gcpE; b2515; JW2499 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
49EATVNQIKALERVGAacetylation[1]
73MDAAEAFKLIKQQVNacetylation[1]
128VVDCARDKNIPIRIGacetylation[1]
143VNAGSLEKDLQEKYGacetylation[1]
148LEKDLQEKYGEPTPQacetylation[1]
177RLNFDQFKVSVKASDacetylation[1]
197ESYRLLAKQIDQPLHacetylation[1]
256KVGFDILKSLRIRSRacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. 
Sequence Annotation
 METAL 270 270 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 273 273 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 305 305 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 312 312 Iron-sulfur (4Fe-4S) (By similarity).  
Keyword
 4Fe-4S; Complete proteome; Iron; Iron-sulfur; Isoprene biosynthesis; Metal-binding; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 372 AA 
Protein Sequence
MHNQAPIQRR KSTRIYVGNV PIGDGAPIAV QSMTNTRTTD VEATVNQIKA LERVGADIVR 60
VSVPTMDAAE AFKLIKQQVN VPLVADIHFD YRIALKVAEY GVDCLRINPG NIGNEERIRM 120
VVDCARDKNI PIRIGVNAGS LEKDLQEKYG EPTPQALLES AMRHVDHLDR LNFDQFKVSV 180
KASDVFLAVE SYRLLAKQID QPLHLGITEA GGARSGAVKS AIGLGLLLSE GIGDTLRVSL 240
AADPVEEIKV GFDILKSLRI RSRGINFIAC PTCSRQEFDV IGTVNALEQR LEDIITPMDV 300
SIIGCVVNGP GEALVSTLGV TGGNKKSGLY EDGVRKDRLD NNDMIDQLEA RIRAKASQLD 360
EARRIDVQQV EK 372 
Gene Ontology
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:HAMAP.
 GO:0046429; F:4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity; IDA:EcoCyc.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0019288; P:isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway; IEA:UniProtKB-UniPathway.
 GO:0016114; P:terpenoid biosynthetic process; IEA:HAMAP.
 GO:0006744; P:ubiquinone biosynthetic process; IDA:EcoCyc. 
Interpro
 IPR013785; Aldolase_TIM.
 IPR011005; Dihydropteroate_synth-like.
 IPR016425; IspG_bac.
 IPR004588; IspG_bac-typ. 
Pfam
 PF04551; GcpE 
SMART
  
PROSITE
  
PRINTS