UniProt Accession

 KCRU_RAT; P25809 

Genbank Protein ID

 X59737 

Genbank Nucleotide ID

 CAA42415.1 

Protein Name

 Creatine kinase U-type, mitochondrial 

Protein Synonyms/Alias

 Acidic-type mitochondrial creatine kinase; Mia-CK; Ubiquitous mitochondrial creatine kinase; U-MtCK 

Gene Name

 Ckmt1 

Gene Synonyms/Alias

 Ckmt 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
149	TTDLDASKIRSGYFD	acetylation	[1]
200	VDALSGLKGDLAGRY	acetylation	[1]
200	VDALSGLKGDLAGRY	ubiquitination	[2]
332	LRAGVHVKLPLLSKD	acetylation	[1]
338	VKLPLLSKDSRFPKI	acetylation	[1]
344	SKDSRFPKILENLRL	acetylation	[1]
403	DCERRLEKGQDIRIP	acetylation	[1]
417	PPPLVHGKH******	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 

Functional Description

 Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. 

Sequence Annotation

 DOMAIN 46 132 Phosphagen kinase N-terminal.
 DOMAIN 159 401 Phosphagen kinase C-terminal.
 NP_BIND 162 166 ATP (By similarity).
 NP_BIND 354 359 ATP (By similarity).
 REGION 40 64 Cardiolipin-binding (By similarity).
 BINDING 225 225 ATP (By similarity).
 BINDING 270 270 ATP (By similarity).
 BINDING 326 326 ATP (By similarity).
 BINDING 369 369 ATP (By similarity).
 MOD_RES 154 154 Phosphotyrosine (By similarity).
 MOD_RES 313 313 Nitrated tyrosine (By similarity).  

Keyword

 ATP-binding; Complete proteome; Direct protein sequencing; Kinase; Membrane; Mitochondrion; Mitochondrion inner membrane; Nitration; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 418 AA 

Protein Sequence

Gene Ontology

 GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; TAS:RGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004111; F:creatine kinase activity; TAS:RGD.
 GO:0046314; P:phosphocreatine biosynthetic process; TAS:RGD. 

Interpro

 IPR000749; ATP-guanido_PTrfase.
 IPR022415; ATP-guanido_PTrfase_AS.
 IPR022414; ATP-guanido_PTrfase_cat.
 IPR022413; ATP-guanido_PTrfase_N.
 IPR014746; Gln_synth/guanido_kin_cat_dom. 

Pfam

 PF00217; ATP-gua_Ptrans
 PF02807; ATP-gua_PtransN 

SMART

  

PROSITE

 PS00112; PHOSPHAGEN_KINASE
 PS51510; PHOSPHAGEN_KINASE_C
 PS51509; PHOSPHAGEN_KINASE_N 

PRINTS