CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037351
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Malate dehydrogenase, mitochondrial 
Protein Synonyms/Alias
  
Gene Name
 MDH2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
26TSAQNNAKVAVLGASubiquitination[1]
45QPLSLLLKNSPLVSRubiquitination[1]
74DLSHIETKAAVKGYLacetylation[2]
74DLSHIETKAAVKGYLubiquitination[1, 3]
78IETKAAVKGYLGPEQubiquitination[4, 5, 6]
91EQLPDCLKGCDVVVIacetylation[2, 6]
91EQLPDCLKGCDVVVIubiquitination[5]
161VIGGHAGKTIIPLISubiquitination[5]
173LISQCTPKVDFPQDQacetylation[2]
197EAGTEVVKAKAGAGSacetylation[6]
197EAGTEVVKAKAGAGSubiquitination[1, 3, 5, 7]
199GTEVVKAKAGAGSATubiquitination[1, 3, 5, 8]
254STPLLLGKKGIEKNLacetylation[2]
254STPLLLGKKGIEKNLubiquitination[1]
259LGKKGIEKNLGIGKVacetylation[2, 6]
259LGKKGIEKNLGIGKVubiquitination[4]
265EKNLGIGKVSSFEEKacetylation[2, 6]
265EKNLGIGKVSSFEEKubiquitination[1, 3, 5]
272KVSSFEEKMISDAIPacetylation[2, 6, 9]
272KVSSFEEKMISDAIPubiquitination[1, 3, 5]
282SDAIPELKASIKKGEubiquitination[1, 5]
287ELKASIKKGEDFVKTacetylation[9]
293KKGEDFVKTLK****acetylation[2, 6, 9]
293KKGEDFVKTLK****ubiquitination[5]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [8] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [9] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 296 AA 
Protein Sequence
MLSALARPAS AALRRSFSTS AQNNAKVAVL GASGGIGQPL SLLLKNSPLV SRLTLYDIAH 60
TPGVAADLSH IETKAAVKGY LGPEQLPDCL KGCDVVVIPA GVPRKPGMTR DDLFNTNATI 120
VATLTAACAQ HCPEAMICVI ANPGLDPARV NVPVIGGHAG KTIIPLISQC TPKVDFPQDQ 180
LTALTGRIQE AGTEVVKAKA GAGSATLSMA YAGARFVFSL VDAMNGKEGV VECSFVKSQE 240
TECTYFSTPL LLGKKGIEKN LGIGKVSSFE EKMISDAIPE LKASIKKGED FVKTLK 296 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0030060; F:L-malate dehydrogenase activity; IEA:InterPro.
 GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro.
 GO:0006108; P:malate metabolic process; IEA:InterPro. 
Interpro
 IPR001557; L-lactate/malate_DH.
 IPR022383; Lactate/malate_DH_C.
 IPR001236; Lactate/malate_DH_N.
 IPR015955; Lactate_DH/Glyco_Ohase_4_C.
 IPR010097; Malate_DH_type1.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF02866; Ldh_1_C
 PF00056; Ldh_1_N 
SMART
  
PROSITE
  
PRINTS