UniProt Accession

 PFD3_HUMAN; P61758; B2R8L5; O55228; Q15765; Q5JT81; Q86X96 

Genbank Protein ID

 U96759; Y17394; AK313420; BT019604; AL356738; BC046094; U56833 

Genbank Nucleotide ID

 AAC23907.1; CAA76761.1; BAG36212.1; AAV38411.1; CAI41469.1; AAH46094.1; AAC50617.1 

Protein Name

 Prefoldin subunit 3 

Protein Synonyms/Alias

 HIBBJ46; Von Hippel-Lindau-binding protein 1; VBP-1; VHL-binding protein 1 

Gene Name

 VBP1 

Gene Synonyms/Alias

 PFDN3 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
5	***MAAVKDSCGKGE	acetylation	[1]
5	***MAAVKDSCGKGE	ubiquitination	[1, 2]
10	AVKDSCGKGEMATGN	ubiquitination	[2]
59	KLDEQYQKYKFMELN	acetylation	[3]
59	KLDEQYQKYKFMELN	ubiquitination	[2, 4, 5]
61	DEQYQKYKFMELNLA	ubiquitination	[2, 6]
70	MELNLAQKKRRLKGQ	ubiquitination	[4, 5, 6]
71	ELNLAQKKRRLKGQI	ubiquitination	[2]
82	KGQIPEIKQTLEILK	ubiquitination	[2, 4, 6]
89	KQTLEILKYMQKKKE	ubiquitination	[2, 6]
95	LKYMQKKKESTNSME	ubiquitination	[6]
185	RVYNWDVKRRNKDDS	ubiquitination	[2, 6]
189	WDVKRRNKDDSTKNK	ubiquitination	[7]

Reference

 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. 

Sequence Annotation

 MOD_RES 59 59 N6-acetyllysine.  

Keyword

 Acetylation; Chaperone; Complete proteome; Cytoplasm; Nucleus; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 197 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0016272; C:prefoldin complex; IEA:InterPro.
 GO:0051084; P:'de novo' posttranslational protein folding; TAS:Reactome. 

Interpro

 IPR009053; Prefoldin.
 IPR016655; Prefoldin_su-3.
 IPR004127; Prefoldin_subunit. 

Pfam

 PF02996; Prefoldin 

SMART

  

PROSITE

  

PRINTS