| Tag | Content |
|---|
| CPLM ID | CPLM-011799 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | mRNA-binding protein PUF2 |
Protein Synonyms/Alias | Pumilio homology domain family member 2 |
Gene Name | PUF2 |
Gene Synonyms/Alias | YPR042C; YP3085.06c; YP9499.01 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 635 | WVCQKIIKMANTPRQ | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] |
Functional Description | RNA-binding protein involved in post-transcriptional regulation. Negatively regulates expression of COX17 by binding to the 3'-UTR of COX17 mRNA. Promotes decay of COX17 mRNA by enhancing its rate of deadenylation and subsequent turnover. Predominantly binds to mRNAs encoding membrane-associated proteins with roles in transmembrane transport and vesicular trafficking. |
Sequence Annotation | DOMAIN 316 402 RRM.
DOMAIN 511 872 PUM-HD.
REPEAT 574 611 Pumilio 1.
REPEAT 612 647 Pumilio 2.
REPEAT 649 683 Pumilio 3.
REPEAT 684 719 Pumilio 4.
REPEAT 722 758 Pumilio 5.
REPEAT 760 800 Pumilio 6.
MOD_RES 72 72 Phosphoserine.
MOD_RES 198 198 Phosphoserine.
MOD_RES 872 872 Phosphoserine.
MOD_RES 876 876 Phosphoserine. |
Keyword | Complete proteome; Cytoplasm; Phosphoprotein; Reference proteome; Repeat; RNA-binding. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1075 AA |
Protein Sequence | MDNKRLYNGN LSNIPEVIDP GITIPIYEED IRNDTRMNTN ARSVRVSDKR GRSSSTSPQK 60
IGSYRTRAGR FSDTLTNLLP SISAKLHHSK KSTPVVVVPP TSSTPDSLNS TTYAPRVSSD 120
SFTVATPLSL QSTTTRTRTR NNTVSSQITA SSSLTTDVGN ATSANIWSAN AESNTSSSPL 180
FDYPLATSYF EPLTRFKSTD NYTLPQTAQL NSFLEKNGNP NIWSSAGNSN TDHLNTPIVN 240
RQRSQSQSTT NRVYTDAPYY QQPAQNYQVQ VPPRVPKSTS ISPVILDDVD PASINWITAN 300
QKVPLVNQIS ALLPTNTISI SNVFPLQPTQ QHQQNAVNLT STSLATLCSQ YGKVLSARTL 360
RGLNMALVEF STVESAICAL EALQGKELSK VGAPSTVSFA RVLPMYEQPL NVNGFNNTPK 420
QPLLQEQLNH GVLNYQLQQS LQQPELQQQP TSFNQPNLTY CNPTQNLSHL QLSSNENEPY 480
PFPLPPPSLS DSKKDILHTI SSFKLEYDHL ELNHLLQNAL KNKGVSDTNY FGPLPEHNSK 540
VPKRKDTFDA PKLRELRKQF DSNSLSTIEM EQLAIVMLDQ LPELSSDYLG NTVIQKLFEN 600
SSNIIRDIML RKCNKYLTSM GVHKNGTWVC QKIIKMANTP RQINLVTSGV SDYCTPLFND 660
QFGNYVIQGI LKFGFPWNSF IFESVLSHFW TIVQNRYGSR AVRACLEADS IITQCQLLTI 720
TSLIIVLSPY LATDTNGTLL ITWLLDTCTL PNKNLILCDK LVNKNLVKLC CHKLGSLTVL 780
KILNLRGGEE EALSKNKIIH AIFDGPISSD SILFQILDEG NYGPTFIYKV LTSRILDNSV 840
RDEAITKIRQ LILNSNINLQ SRQLLEEVGL SSAGISPKQS SKNHRKQHPQ GFHSPGRARG 900
VSVSSVRSSN SRHNSVIQMN NAGPTPALNF NPAPMSEINS YFNNQQVVYS GNQNQNQNGN 960
SNGLDELNSQ FDSFRIANGT NLSLPIVNLP NVSNNNNNYN NSGYSSQMNP LSRSVSHNNN 1020
NNTNNYNNND NDNNNNNNNN NNNNNNNNNN NNNSNNSNNN NNNDTSLYRY RSYGY 1075 |
Gene Ontology | GO:0005737; C:cytoplasm; IDA:SGD. GO:0003729; F:mRNA binding; IDA:SGD. GO:0000166; F:nucleotide binding; IEA:InterPro. GO:0000288; P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay; IGI:SGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |