UniProt Accession

 PSDE_MOUSE; O35593; Q3UB50; Q9CZY6 

Genbank Protein ID

 Y13071; AK012013; AK151104; AK151733; BC003742 

Genbank Nucleotide ID

 CAA73514.1; BAB27974.1; BAE30114.1; BAE30648.1; AAH03742.1 

Protein Name

 26S proteasome non-ATPase regulatory subunit 14 

Protein Synonyms/Alias

 26S proteasome regulatory subunit RPN11; MAD1 

Gene Name

 Psmd14 

Gene Synonyms/Alias

 Pad1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
152	VDPIQSVKGKVVIDA	ubiquitination	[1]
246	KHNESVVKEMLELAK	ubiquitination	[1]
257	ELAKNYNKAVEEEDK	ubiquitination	[1]
273	TPEQLAIKNVGKQDP	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. Plays a role in response to double-strand breaks (DSBs): acts as a regulator of non-homologous end joining (NHEJ) by cleaving 'Lys-63'-linked polyubiquitin, thereby promoting retention of JMJD2A/KDM4A on chromatin and restricting TP53BP1 accumulation. Also involved in homologous recombination repair by promoting RAD51 loading (By similarity). 

Sequence Annotation

 DOMAIN 26 139 MPN.
 MOTIF 113 126 JAMM motif.
 METAL 113 113 Zinc; catalytic (By similarity).
 METAL 115 115 Zinc; catalytic (By similarity).
 METAL 126 126 Zinc; catalytic (By similarity).
 MOD_RES 150 150 Phosphoserine (By similarity).
 MOD_RES 224 224 Phosphoserine (By similarity).  

Keyword

 Complete proteome; Direct protein sequencing; DNA damage; DNA repair; Hydrolase; Metal-binding; Metalloprotease; Phosphoprotein; Protease; Proteasome; Reference proteome; Ubl conjugation pathway; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 310 AA 

Protein Sequence

Gene Ontology

 GO:0008541; C:proteasome regulatory particle, lid subcomplex; ISS:UniProtKB.
 GO:0061133; F:endopeptidase activator activity; IEA:Compara.
 GO:0004175; F:endopeptidase activity; ISS:MGI.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
 GO:0004221; F:ubiquitin thiolesterase activity; ISS:UniProtKB.
 GO:0000724; P:double-strand break repair via homologous recombination; ISS:UniProtKB.
 GO:0006303; P:double-strand break repair via nonhomologous end joining; ISS:UniProtKB.
 GO:0070536; P:protein K63-linked deubiquitination; ISS:UniProtKB.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
 GO:0061136; P:regulation of proteasomal protein catabolic process; IEA:Compara. 

Interpro

 IPR000555; JAB1_Mov34_MPN_PAD1.
 IPR024969; Rpn11/EIF3F_C. 

Pfam

 PF01398; JAB
 PF13012; MitMem_reg 

SMART

 SM00232; JAB_MPN 

PROSITE

  

PRINTS