UniProt Accession

 ECE1_HUMAN; P42892; A8K3P1; B4E291; Q14217; Q17RN5; Q2Z2K8; Q58GE7; Q5THM5; Q5THM7; Q5THM8; Q9UJQ6; Q9UPF4; Q9UPM4; Q9Y501 

Genbank Protein ID

 D49471; D43698; X91922; X91923; X91924; X91925; X91926; X91927; X91928; X91929; X91930; X91931; X91932; X91933; X91934; X91935; X91936; X91937; X91938; X91939; X91923; X91924; X91925; X91926; X91927; X91928; X91929; X91930; X91931; X91932; X91933; X91934; X91935; X91936; X91937; X91938; X91939; AB031742; AK290656; AK304167; AY953519; AL031005; AL031005; AL031728; AL031005; AL031728; AL031728; AL031728; AL031005; CH471134; CH471134; BC117256; BC126257; AJ130828; X98272; Z35307; AF018034 

Genbank Nucleotide ID

 BAA08442.1; BAA07800.1; CAA63015.1; CAA63015.1; CAA63015.1; CAA63015.1; CAA63015.1; CAA63015.1; CAA63015.1; CAA63015.1; CAA63015.1; CAA63015.1; CAA63015.1; CAA63015.1; CAA63015.1; CAA63015.1; CAA63015.1; CAA63015.1; CAA63015.1; CAA63015.1; CAA63016.1; CAA63016.1; CAA63016.1; CAA63016.1; CAA63016.1; CAA63016.1; CAA63016.1; CAA63016.1; CAA63016.1; CAA63016.1; CAA63016.1; CAA63016.1; CAA63016.1; CAA63016.1; CAA63016.1; CAA63016.1; CAA63016.1; BAA83687.1; BAF83345.1; BAG65053.1; AAX35820.1; CAI20195.1; CAI20192.1; CAI20192.1; CAI20194.1; CAI20194.1; CAB52285.2; CAI19064.1; CAI19064.1; EAW94959.1; EAW94964.1; AAI17257.1; AAI26258.1; CAB46443.1; CAA66922.1; CAA84548.1; AAD21221.1 

Protein Name

 Endothelin-converting enzyme 1 

Protein Synonyms/Alias

 ECE-1 

Gene Name

 ECE1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
197	RIEELRAKPLMELIE	ubiquitination	[1]
271	SRDYYLNKTENEKVL	ubiquitination	[2]
472	TEIILEIKKAFEESL	ubiquitination	[2]
496	TRKSAKEKADAIYNM	ubiquitination	[2]
639	NSSVEAFKRQTECMV	ubiquitination	[2]
676	IADNGGLKAAYRAYQ	ubiquitination	[1, 2]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Converts big endothelin-1 to endothelin-1. 

Sequence Annotation

 ACT_SITE 608 608
 ACT_SITE 671 671 Proton donor (By similarity).
 METAL 607 607 Zinc; catalytic.
 METAL 611 611 Zinc; catalytic.
 METAL 667 667 Zinc; catalytic.
 MOD_RES 25 25 Phosphothreonine.
 CARBOHYD 166 166 N-linked (GlcNAc...).
 CARBOHYD 187 187 N-linked (GlcNAc...) (Potential).
 CARBOHYD 210 210 N-linked (GlcNAc...).
 CARBOHYD 270 270 N-linked (GlcNAc...).
 CARBOHYD 316 316 N-linked (GlcNAc...).
 CARBOHYD 362 362 N-linked (GlcNAc...).
 CARBOHYD 383 383 N-linked (GlcNAc...).
 CARBOHYD 539 539 N-linked (GlcNAc...) (Potential).
 CARBOHYD 632 632 N-linked (GlcNAc...) (Potential).
 CARBOHYD 651 651 N-linked (GlcNAc...) (Potential).
 DISULFID 428 428 Interchain.  

Keyword

 3D-structure; Alternative splicing; Cell membrane; Complete proteome; Disease mutation; Disulfide bond; Glycoprotein; Hirschsprung disease; Hydrolase; Membrane; Metal-binding; Metalloprotease; Phosphoprotein; Polymorphism; Protease; Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 770 AA 

Protein Sequence

Gene Ontology

 GO:0005769; C:early endosome; IDA:BHF-UCL.
 GO:0009897; C:external side of plasma membrane; IDA:BHF-UCL.
 GO:0016021; C:integral to membrane; IDA:BHF-UCL.
 GO:0031302; C:intrinsic to endosome membrane; TAS:BHF-UCL.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:BHF-UCL.
 GO:0005886; C:plasma membrane; IDA:BHF-UCL.
 GO:0033093; C:Weibel-Palade body; IDA:BHF-UCL.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004222; F:metalloendopeptidase activity; ISS:BHF-UCL.
 GO:0017046; F:peptide hormone binding; IC:BHF-UCL.
 GO:0010815; P:bradykinin catabolic process; IDA:BHF-UCL.
 GO:0010816; P:calcitonin catabolic process; IDA:BHF-UCL.
 GO:0043583; P:ear development; IMP:BHF-UCL.
 GO:0042733; P:embryonic digit morphogenesis; IMP:BHF-UCL.
 GO:0034959; P:endothelin maturation; IDA:BHF-UCL.
 GO:0007507; P:heart development; IMP:BHF-UCL.
 GO:0060037; P:pharyngeal system development; IEA:Compara.
 GO:0001921; P:positive regulation of receptor recycling; IMP:BHF-UCL.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
 GO:0003100; P:regulation of systemic arterial blood pressure by endothelin; IC:BHF-UCL.
 GO:0019229; P:regulation of vasoconstriction; IC:BHF-UCL.
 GO:0010814; P:substance P catabolic process; IDA:BHF-UCL. 

Interpro

 IPR024079; MetalloPept_cat_dom.
 IPR000718; Peptidase_M13.
 IPR018497; Peptidase_M13_C.
 IPR008753; Peptidase_M13_N. 

Pfam

 PF01431; Peptidase_M13
 PF05649; Peptidase_M13_N 

SMART

  

PROSITE

 PS00142; ZINC_PROTEASE 

PRINTS

 PR00786; NEPRILYSIN.