| Tag | Content |
|---|
| CPLM ID | CPLM-010944 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Transcriptional adapter 2 |
Protein Synonyms/Alias | |
Gene Name | ADA2 |
Gene Synonyms/Alias | YDR448W; D9461.33 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 299 | TTLEAGLKYERDKQA | acetylation | [1] |
|
Reference | [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae. Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C. Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [ PMID: 22865919] |
Functional Description | Functions as component of the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and ADA. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus. ADA preferentially acetylates nucleosomal histones H3 (to form H3K14ac and H3K18ac) and H2B. |
Sequence Annotation | DOMAIN 60 112 SANT.
DOMAIN 349 434 SWIRM.
ZN_FING 1 48 ZZ-type. |
Keyword | 3D-structure; Complete proteome; Metal-binding; Nucleus; Reference proteome; Transcription; Transcription regulation; Zinc; Zinc-finger. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 434 AA |
Protein Sequence | MSNKFHCDVC SADCTNRVRV SCAICPEYDL CVPCFSQGSY TGKHRPYHDY RIIETNSYPI 60
LCPDWGADEE LQLIKGAQTL GLGNWQDIAD HIGSRGKEEV KEHYLKYYLE SKYYPIPDIT 120
QNIHVPQDEF LEQRRHRIES FRERPLEPPR KPMASVPSCH EVQGFMPGRL EFETEFENEA 180
EGPVKDMVFE PDDQPLDIEL KFAILDIYNS RLTTRAEKKR LLFENHLMDY RKLQAIDKKR 240
SKEAKELYNR IKPFARVMTA QDFEEFSKDI LEELHCRARI QQLQEWRSNG LTTLEAGLKY 300
ERDKQARISS FEKFGASTAA SLSEGNSRYR SNSAHRSNAE YSQNYSENGG RKKNMTISDI 360
QHAPDYALLS NDEQQLCIQL KILPKPYLVL KEVMFRELLK TGGNLSKSAC RELLNIDPIK 420
ANRIYDFFQS QNWM 434 |
Gene Ontology | GO:0005671; C:Ada2/Gcn5/Ada3 transcription activator complex; IDA:SGD. GO:0000124; C:SAGA complex; IDA:SGD. GO:0046695; C:SLIK (SAGA-like) complex; IDA:SGD. GO:0003682; F:chromatin binding; IDA:SGD. GO:0003677; F:DNA binding; IEA:InterPro. GO:0001786; F:phosphatidylserine binding; IDA:SGD. GO:0003713; F:transcription coactivator activity; IDA:SGD. GO:0008270; F:zinc ion binding; IEA:InterPro. GO:0000183; P:chromatin silencing at rDNA; IMP:SGD. GO:0006348; P:chromatin silencing at telomere; IMP:SGD. GO:0016573; P:histone acetylation; IDA:GOC. GO:0035066; P:positive regulation of histone acetylation; IMP:SGD. GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IMP:SGD. GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. |
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PROSITE | |
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