CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012726
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Diacylglycerol kinase delta 
Protein Synonyms/Alias
 DAG kinase delta; 130 kDa diacylglycerol kinase; Diglyceride kinase delta; DGK-delta 
Gene Name
 DGKD 
Gene Synonyms/Alias
 KIAA0145 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
254AKCTVCDKTCGSVLRubiquitination[1, 2]
454SVMAYEAKLPRQASSubiquitination[1]
524RVGKAYEKTTESSEEubiquitination[1]
718GLPALNTKILYPNVRubiquitination[1, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 May function as signaling molecule. 
Sequence Annotation
 DOMAIN 53 146 PH.
 DOMAIN 317 451 DAGKc.
 DOMAIN 1145 1208 SAM.
 ZN_FING 163 213 Phorbol-ester/DAG-type 1.
 ZN_FING 235 286 Phorbol-ester/DAG-type 2.  
Keyword
 3D-structure; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Endocytosis; Kinase; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Protein transport; Reference proteome; Repeat; Transferase; Transport; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1214 AA 
Protein Sequence
MAAAAGAPPP GPPQPPPPPP PEESSDSEPE AEPGSPQKLI RKVSTSGQIR QKTIIKEGML 60
TKQNNSFQRS KRRYFKLRGR TLYYAKTAKS IIFDEVDLTD ASVAESSTKN VNNSFTVITP 120
CRKLILCADN RKEMEDWIAA LKTVQNREHF EPTQYSMDHF SGMHNWYACS HARPTYCNVC 180
REALSGVTSH GLSCEVCKFK AHKRCAVRAT NNCKWTTLAS IGKDIIEDAD GIAMPHQWLE 240
GNLPVSAKCT VCDKTCGSVL RLQDWRCLWC KAMVHTSCKE SLLTKCPLGL CKVSVIPPTA 300
LNSIDSDGFW KASCPPSCTS PLLVFVNSKS GDNQGVKFLR RFKQLLNPAQ VFDLMNGGPH 360
LGLRLFQKFD TFRILVCGGD GSVGWVLSEI DSLNLHKQCQ LGVLPLGTGN DLARVLGWGS 420
ACDDDTQLPQ ILEKLERAST KMLDRWSVMA YEAKLPRQAS SSTVTEDFSE DSEVQQILFY 480
EDSVAAHLSK ILTSDQHSVV ISSAKVLCET VKDFVARVGK AYEKTTESSE ESEVMAKKCS 540
VLKEKLDSLL KTLDDESQAS SSLPNPPPTI AEEAEDGDGS GSICGSTGDR LVASACPARP 600
QIFRPREQLM LRANSLKKAI RQIIEHTEKA VDEQNAQTQE QEGFVLGLSE SEEKMDHRVC 660
PPLSHSESFG VPKGRSQRKV SKSPCEKLIS KGSLSLGSSA SLPPQPGSRD GLPALNTKIL 720
YPNVRAGMSG SLPGGSVISR LLINADPFNS EPETLEYYTE KCVMNNYFGI GLDAKISLDF 780
NNKRDEHPEK CRSRTKNMMW YGVLGTKELL HRTYKNLEQK VLLECDGRPI PLPSLQGIAV 840
LNIPSYAGGT NFWGGTKEDD TFAAPSFDDK ILEVVAVFGS MQMAVSRVIR LQHHRIAQCR 900
TVKISILGDE GVPVQVDGEA WVQPPGYIRI VHKNRAQTLT RDRAFESTLK SWEDKQKCEL 960
PRPPSCSLHP EMLSEEEATQ MDQFGQAAGV LIHSIREIAQ SHRDMEQELA HAVNASSKSM 1020
DRVYGKPRTT EGLNCSFVLE MVNNFRALRS ETELLLSGKM ALQLDPPQKE QLGSALAEMD 1080
RQLRRLADTP WLCQSAEPGD EESVMLDLAK RSRSGKFRLV TKFKKEKNNK NKEAHSSLGA 1140
PVHLWGTEEV AAWLEHLSLC EYKDIFTRHD IRGSELLHLE RRDLKDLGVT KVGHMKRILC 1200
GIKELSRSAP AVEA 1214 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0016023; C:cytoplasmic membrane-bounded vesicle; IDA:MGI.
 GO:0005886; C:plasma membrane; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0019992; F:diacylglycerol binding; NAS:UniProtKB.
 GO:0004143; F:diacylglycerol kinase activity; TAS:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0046982; F:protein heterodimerization activity; IDA:MGI.
 GO:0042803; F:protein homodimerization activity; IDA:MGI.
 GO:0016049; P:cell growth; NAS:UniProtKB.
 GO:0046339; P:diacylglycerol metabolic process; NAS:UniProtKB.
 GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; NAS:UniProtKB.
 GO:0007275; P:multicellular organismal development; NAS:UniProtKB.
 GO:0030168; P:platelet activation; TAS:Reactome.
 GO:0051260; P:protein homooligomerization; IDA:UniProtKB.
 GO:0007205; P:protein kinase C-activating G-protein coupled receptor signaling pathway; NAS:UniProtKB.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0010033; P:response to organic substance; IDA:UniProtKB.
 GO:0019932; P:second-messenger-mediated signaling; NAS:UniProtKB. 
Interpro
 IPR000756; Diacylglycerol_kin_accessory.
 IPR001206; Diacylglycerol_kinase_cat_dom.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd.
 IPR001660; SAM.
 IPR013761; SAM/pointed.
 IPR011510; SAM_2. 
Pfam
 PF00130; C1_1
 PF00609; DAGK_acc
 PF00781; DAGK_cat
 PF00169; PH
 PF07647; SAM_2 
SMART
 SM00109; C1
 SM00045; DAGKa
 SM00046; DAGKc
 SM00233; PH
 SM00454; SAM 
PROSITE
 PS50146; DAGK
 PS50003; PH_DOMAIN
 PS50105; SAM_DOMAIN
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 
PRINTS